杭锦旗小花棘豆种群苦马豆素提取测定研究

小花棘豆是豆科棘豆属植物,俗称醉马草,被认为是对家畜危害最大的一类有毒植物,有毒成分是其内所含的苦马豆素。本研究对内蒙古杭锦旗3个种群的41株小花棘豆中所含苦马豆素用化学法进行提取,用高效气相色谱技术进行了含量测定分析。结果显示:不同种群、不同植株苦马豆素含量存在差异。HJ1种群15株植物的SW平均含量为19.84μg/g,含量范围是0.28～172.70μg/g;HJ2种群14株植物的SW平均含量为109.14μg/g,含量范围是1.50～305.30μg/g;HJ3种群12株植物的SW平均含量为91.51μg/g,含量范围是0.36～222.22μg/g。

Extraction and Determination of Swainsonine from Oxytropis glabra Populations in Hangjin Banner

Oxytropis glabra belongs to leguminous species,commonly known as the drunken horse grass.It is often considered one of the most harmful plants to livestock.The toxic ingredient is swainsonine.In this study,swainsonine was extracted by chemical method and determined by Gas Chromatography from 41 plant samples of Oxytropics glabra collected from three populations in Hangjin Banner,Inner Mongolia.The results indicated that swainsonine contents changed among different populations and different single plant sample.The average content of swainsonine in HJ1 population was 19.84 μg/g,and the concentration ranged from 0.28 μg/g to 172.70 μg/g.The average content of swainsonine in HJ2 population was 109.14 μg/g,and the concentration ranged from 1.50 μg/g to 305.30 μg/g.The average content of swainsonine in HJ3 population was 91.51 μg/g,and the concentration ranged from 0.36 μg/g to 222.22 μg/g.