| 大花蕙兰的嫩根与根兜组织快繁技术研究 |
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| 引用本文： | 赵颖,刘平生,张南.大花蕙兰的嫩根与根兜组织快繁技术研究[J].内蒙古林业科技,2010,36(3):48-50. |
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| 作者姓名： | 赵颖 刘平生 张南 |
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| 作者单位： | [1]内蒙古自治区林业科学研究院,内蒙古呼和浩特010010 [2]内蒙古林木种苗示范基地,内蒙古呼和浩特010000 |
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| 摘 要： | 取大花蕙兰的的嫩根和根兜为外植体在MS培养基上用不同浓度的细胞分裂素BA和生长素NAA进行了研究。结果表明：根兜的分化率比嫩根高,而最适合的培养基配方为MS＋BA1.5 mg/L＋NAA0.5 mg/L＋蔗糖30 g/L＋琼脂8 g/L。可直接分化成小苗,增殖的同时可获得健壮的再生植株。最适的生根培养基为：MS＋NAA0.3 mg/L＋活性碳1.0/L＋蔗糖25 g/L＋琼脂7 g/L。
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| 关 键 词： | 大花蕙兰 嫩根 根兜 组培 细胞分裂素 生长素 |
Tissue Rapid Propagation by Tender Root and Rhizome of Cymbidium hybridium |
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| ZHAO Ying,LIU Ping-sheng,ZHANG Nan.Tissue Rapid Propagation by Tender Root and Rhizome of Cymbidium hybridium[J].Inner Mongolia Forestry Science and Technology,2010,36(3):48-50. |
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| Authors: | ZHAO Ying LIU Ping-sheng ZHANG Nan |
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| Institution: | 1.Inner Mongolia Academy of Forestry Science,Hohhot 010010,China;2.Inner Mongolia Tree Seed and Seedling Station,Hohhot 010000,China) |
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| Abstract: | Taking tender root and rhizome of Cymbidium hybridium as explant,the research is made with cytokinin BA and auxin NAA at different concentrations.The results show： the differentiation rate of rhizome is higher than tender root,which the most suitable formula is MS＋BA1.5 mg/L＋NAA0.5 mg/L＋sucrose 30 g/L＋agar 8 g/L.It can differentiate into seedlins and regenerated plants.The most suitable rooting medium is MS＋NAA0.3 mg/L＋active carbon 1.0/L＋sucrose 25 g/L＋agar 7 g/L. |
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| Keywords: | Cymbidium hybridium tender root tissue cytokinin auxin |
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