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火焰卫矛组织培养及植株再生研究
引用本文:祖庆学,赵德刚.火焰卫矛组织培养及植株再生研究[J].福建林业科技,2008,35(3).
作者姓名:祖庆学  赵德刚
作者单位:贵州大学生命科学学院贵州省农业生物工程重点实验室,贵州,贵阳,550025
基金项目:贵州省优秀科技教育人才省长基金,贵州省高层次人才专项基金 
摘    要:以火焰卫矛成熟胚培养长出的子叶为外植体进行组织培养及植株再生研究,建立了火焰卫矛再生体系,成熟胚培养基为WPM+TDZ 0.5 mg.L-1;子叶愈伤组织诱导和不定芽分化培养基为WPM+6-BA 6 mg.L-1+IBA 0.2 mg.L-1;生根培养基为1/2MS+NAA 0.2 mg.L-1+活性碳0.5 g.L-1。

关 键 词:火焰卫矛  子叶  组织培养

Tissue culture and plantlet regeneration of Euonymus alatus'compacta'
ZU Qing-xue,ZHAO De-gang.Tissue culture and plantlet regeneration of Euonymus alatus''compacta''[J].Journal of Fujian Forestry Science and Technology,2008,35(3).
Authors:ZU Qing-xue  ZHAO De-gang
Abstract:The cotyledon from mature embryo of Euonymus alatus 'compacta' was used as the explant for studying the tissue culture and the plantlet regeneration.The results indicated that the medium for mature embryo was WPM+TDZ 0.5 mg·L-1,the medium for callus induction from the cotyledon.and the medium for bud differentiation were WPM+6-BA 6 mg·L-1+IBA 0.2 mg·L-1,the medium for rooting of adventitious buds was 1/2MS+NAA 0.2 mg·L-1+active carbon 0.5 g·L-1.These provide fundamentals for rapid propagation and genetic engineering of Euonymus alatus 'compacta'.
Keywords:Euonymus alatus 'compacta'  cotyledon  tissue culture
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