| 椰心叶甲病原细菌生物学初步研究 |
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| 引用本文： | 孙蕊芬,赵益,朱麟.椰心叶甲病原细菌生物学初步研究[J].福建林业科技,2011,38(1). |
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| 作者姓名： | 孙蕊芬 赵益 朱麟 |
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| 作者单位： | 海南师范大学生命科学学院海南省热带野生动植物生态学重点实验室,海南,海口,571158 |
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| 基金项目： | 海南省教育厅"椰心叶甲种群生物学研究(hjkj200618)"、"椰心叶甲两种寄生蜂生态适应性研究(hjkj2009-38)"，海南省教育厅高校研究生创新课题"椰心叶甲两种寄生蜂热激蛋白的表达研究(hxwsy 2009-20)"资助 |
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| 摘 要： | 在调查过程中,首次在海南发现了1种感染椰心叶甲幼虫的病原细菌,并在室内条件下对其培养条件和感染性状进行了初步研究,分离得到病原细菌在牛肉膏蛋白胨培养基培养呈朱红色,圆形或放射状,表面光滑有光泽,革兰氏阴性,有荚膜,无芽孢,有鞭毛的好氧短杆菌;在pH 5.0、6.0、7.0、8.0、9.0条件下均能正常生长;适宜温度范围18.0～37.0℃,并在这个温度范围内随温度升高,生长繁殖速度加快。在室内条件下含菌量为7.23×1010 cfu·mL-1的工程菌悬浮液,采用叶片浸渍法饲喂椰心叶甲幼虫,在11 d后致死率为27.65%。被感染椰心叶甲幼虫全身变红,体态完好,内部器官腐烂为脓水,表皮易破,有腐臭味。
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| 关 键 词： | 椰心叶甲 病原细菌 致死率 |
Preliminary Study of A New Pathogenicbacteria of Brontispa longissima(Gestro) |
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| SUN Rui-fen,ZHAO Yi,ZHU Lin.Preliminary Study of A New Pathogenicbacteria of Brontispa longissima(Gestro)[J].Journal of Fujian Forestry Science and Technology,2011,38(1). |
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| Authors: | SUN Rui-fen ZHAO Yi ZHU Lin |
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| Institution: | SUN Rui-fen,ZHAO Yi,ZHU Lin(Hainan Provincial Key Laboratory of Tropical Plant and Animal Ecology,College of Life Sciences,Hainan Normal University,Haikou 571158,Hainan,China) |
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| Abstract: | A pathogenic bacteria infecting Brontispa longissima(Gestro),an alien invasive pest attacking coconut palm plants,was found,and under laboratory conditions,their culture conditions and infection traits were preliminary studied.The bacteria were isolated from the body of Brontispa longissima larvae and the pathogenicity were bioassayed.The colonies showed minium,round shape or actinomorphic,slippery surfaces and glossiness in the BP culture media;the bacterial cell showed negative Gram,a capsule,non-spore,sh... |
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| Keywords: | Brontispa longissima(Gestro) pathogenicbacteria death rate |
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