速生优良杉木组培快繁技术试验

应用正交设计法研究外植体的最佳消毒方式,同时考察了培养基、6-BA、KT、NAA等4个因子对杉木组培苗芽增殖的影响和IBA、NAA、ABT1#、蔗糖等4个因子对组培苗生根培养的影响。结果表明:以茎尖为外植体材料,消毒方式采用70%乙醇处理40 s后无菌水冲洗一遍,再用0.1%升汞溶液处理7 min后用无菌水冲洗5次以上;最佳的增殖培养基是1/2MS+6-BA 0.8 mg·L-1,最适于生根的培养基是MS培养基中+蔗糖30 g.L-1+IBA 1.2 mg·L-1+NAA 0.2 mg·L-1+ABT1#1.5 mg·L-1。

A study on the technique of the superior Chinese fir tissue culture and rapid propagation

The orthogonal design was used to select the optimal method for the explant disinfection.This paper studied the effects of 4 factors,medium,6-BA,KT and NAA,on the bud propagation,and the effects of 4 factors,IBA,NAA,ABT1# and sucrose,on rooting in tissue culture.The result showed that the stem tips should be used for explants;the disinfection method was using 70% alcohol for 40 s then rinsed with autoclaved water once,followed by 0.1% HgCl2 for 7 min then rinsed with autoclaved water 5 times;1/2MS medium supplemented with 6-BA 0.8 mg·L-1 was suitable for its multiplication,and MS supplemented with sucrose 30 g·L-1 + IBA 1.2 mg·L-1 + NAA 0.2 mg.L-1 + ABT1# 1.5 mg·L-1.