| 牡丹愈伤组织诱导和继代培养体系的建立 |
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| 引用本文： | 刘会超,贾文庆.牡丹愈伤组织诱导和继代培养体系的建立[J].福建林业科技,2009,36(2). |
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| 作者姓名： | 刘会超 贾文庆 |
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| 作者单位： | 河南科技学院园林学院,河南,新乡,453003 |
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| 基金项目： | 河南省创新人才工程项目，河南科技学院博士基金，河南科技学院重点项目资金资助项目 |
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| 摘 要： | 以牡丹为材料,研究了不同外植体、激素对愈伤组织诱导和继代增殖的影响,结果表明:①牡丹子叶、胚轴是诱导愈伤组织的最佳外植体;②牡丹子叶愈伤组织诱导的最佳培养基为:MS+Ca2+;③牡丹愈伤组织增殖的最佳培养基为:MS+2,4-D 0.5 mg.L-1+NAA 1.5 mg.L-1+Vc 500 mg.L-1;④在培养基中添加500 mg.L-1Vc或去除Cu2+,具有较好的抗褐化效果。
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| 关 键 词： | 牡丹 愈伤组织诱导 继代培养 褐化 |
Callus Induction and Subculture System Establishment of Peony |
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| LIU Hui-chao,JIA Wen-qing.Callus Induction and Subculture System Establishment of Peony[J].Journal of Fujian Forestry Science and Technology,2009,36(2). |
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| Authors: | LIU Hui-chao JIA Wen-qing |
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| Institution: | LIU Hui-chao,JIA Wen-qing(School of Horticulture and Landscape Architecture,Henan College of Science and Technology,Xinxiang 453003,Henan,China) |
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| Abstract: | The effects of different explants and hormones on callus induction and the generation-continuing multiplication were established with peony,the main results were as follows:(1) cotyledon,hypocotyl were the best explants for the callus induction;(2)MS+Ca2+ was the best medium for the callus induction;(3)MS+0.5 mg·L-1 2,4-D+1.5 mg·L-1 NAA+500 mg·L-1 Vc was the best medium for callus multiplication.The browning of callus could be avoided or decreased to a certain degree when 500 mg·L-1 Vc was added to the mediums,or Cu2+ was cleared in the mediums. |
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| Keywords: | Paeonia suffruticosa callus induction subculture browning |
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