| Effects of Metal Ions and EDTA on Free Radical Reaction Intermediates of Laccase-catalyzed Oxidation of Wood Powder from Szemao Pine |
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| 作者姓名： | ZHOUGuanwu DUANXinfang** CAOYongjian CHENYongsheng CAO Yuanlin** .Division of Wood Protection Research Institute of Wood Industry Chinese Academy ofForestry Beijing P.R.China .Centerfor Brain and CognitiveSciences Institute of Biophysics Chinese AcademyofSciences Beijing P.R. China |
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| 作者单位： | ZHOUGuanwu1，2 DUANXinfang1** CAOYongjian1 CHENYongsheng2 CAO Yuanlin2** 1.Division of Wood Protection，Research Institute of Wood Industry Chinese Academy ofForestry，Beijing 100091，P.R.China 2.Centerfor Brain and CognitiveSciences，Institute of Biophysics Chinese AcademyofSciences，Beijing 100101，P.R. China |
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| 基金项目： | This work was supported by the National Natural Science Foundation of China (30471352). |
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| 摘 要： | INTRODUCTIONLaccase (EC 1.10.3.2) is multicopper oxidase catalyzing oxidation of various phenolic compounds, aromatic amines, and even certain inorganic compounds by a one-electron transfer mechanism. The electron withdrawn from the substrates is transferred via four copper atoms to molecular oxygen (Messerschmidt 1997). Laccase is very common in nature, especially in plants and fungi (Gianfreda et al. 1999). Fungal laccases participate in plant pathogenesis, pigment production and ligni…
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| 关 键 词： | 松树 金属离子 顺磁共振光谱学 木材性能 |
Effects of Metal Ions and EDTA on Free Radical Reaction Intermediates of Laccase-catalyzed Oxidation of Wood Powder from Szemao Pine |
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| ZHOUGuanwu, DUANXinfang** CAOYongjian CHENYongsheng CAO Yuanlin** .Division of Wood Protection,Research Institute of Wood Industry Chinese Academy ofForestry,Beijing ,P.R.China .Centerfor Brain and CognitiveSciences,Institute of Biophysics Chinese AcademyofSciences,Beijing ,P.R. China.Effects of Metal Ions and EDTA on Free Radical Reaction Intermediates of Laccase-catalyzed Oxidation of Wood Powder from Szemao Pine[J].中国林业科技(英文版),2006,5(2):28-32. |
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| Authors: | ZHOU Guanwu DUAN Xinfang CAO Yongjian CHEN Yongsheng CAO Yuanlin |
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| Institution: | [1]Division of Wood Protection Research Institute of Wood Industry Chinese Academy of Forestry, Beijing 100091,P.R.China [2]Center for Brain and Cognitive Sciences Institute of Biophysics Chinese Academy of Sciences, Beijing 100101,P.R. China |
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| Abstract: | Certain activator was expected to be developed to improve the free radical intermediates relative intensity, and thus to enhance the adhesion between wood fibers when fiberboard was made by laccase treated wood fibers. Reactive oxygen species (ROS) was detected in laccase-catalyzed oxidation of wood powder using ESR spin trapping technique. The effects of five metal ions (Cu2+, K+, Fe2+, Mg2+, Fe3+) and EDTA on ROS relative strengths were investigated under the condition of pH 5.6 by electron spin resonance spectrometry. The result shows that Cu2+ slightly activates the free radical reaction, and Fe2+ ion has a significant inhibitive effect on the ROS relative strength in the suspension liquid. There is a marked inhibition of the ROS relative intensity when 0.5 mmol/L EDTA is used. The metal ions that enhance laccase activity may be utilized to increase physical properties of fiberboard made by laccase oxidized wood fibers. |
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| Keywords: | reactive oxygen species laccase Szemao pine (Pinus kesiya var langbianensis) electron spin resonance spectrometry metal ion |
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