| 11个中国梨品种S基因型的鉴定 |
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| 引用本文： | 邓建军,谭晓风,包梅荣,乌云塔娜,张琳,曹玉芬.11个中国梨品种S基因型的鉴定[J].中南林业科技大学学报,2010,30(3). |
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| 作者姓名： | 邓建军 谭晓风 包梅荣 乌云塔娜 张琳 曹玉芬 |
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| 作者单位： | 邓建军,谭晓风,包梅荣,乌云塔娜,张琳,DENG Jian-jun,TAN Xiao-feng,BAO Mei-rong,WUYUN Ta-na,ZHANG Lin(中南林业科技大学,经济林育种与栽培国家林业局重点实验室,湖南,长沙,410004);曹玉芬,CAO Yu-fen(中国农业科学院,果树研究所,辽宁,兴城,125100) |
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| 基金项目： | 国家林业局重点科学技术研究计划林业新技术开发与储备专项 |
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| 摘 要： | 采用梨自交不亲和基因的特异引物"FTQQYQ"和"anti-IIWPNV",对11个梨品种的基因组DNA进行了特异PCR扩增,对扩增片段进行回收、克隆和测序。利用生物信息学软件对各序列进行比对分析,最后确定了各品种的S基因型,即早熟句句、新疆黄梨、绿句句、魁克句句等4个新疆梨品种的S基因型均为S22S28;圆香为S16S15,福安尖把为S16S22,晋蜜梨为S21S28,兰州软儿为SdS12,迟咸丰为S5aS18,红秀2号为S1S12,鸭广梨为S19S30。
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| 关 键 词： | 经济林学 梨 自交不亲和性 S等位基因 S基因型 |
S-genotypes of 11 Chinese pear cultivars |
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| DENG Jian-jun,TAN Xiao-feng,BAO Mei-rong,WUYUN Ta-na,ZHANG Lin,CAO Yu-fen.S-genotypes of 11 Chinese pear cultivars[J].Journal of Central South Forestry University,2010,30(3). |
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| Authors: | DENG Jian-jun TAN Xiao-feng BAO Mei-rong WUYUN Ta-na ZHANG Lin CAO Yu-fen |
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| Institution: | DENG Jian-jun1,TAN Xiao-feng1,BAO Mei-rong1,WUYUN Ta-na1,ZHANG Lin1,CAO Yu-fen2(1.The Key Lab of Non-wood Forest Product of Forestry Ministry,Central South University of Forestry & TechnologyChangsha 410004,Hunan,China,2.The Chinese Academy of Agricultural Aciences of Fruit Tree Research Institute,Xingcheng 121600,Liaoning,China) |
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| Abstract: | Using a pair of primers specific to pear S-RNase,PCR amplification from genomic DNAs of eleven pear cultivars were carried out.Then the PCR products were extracted,cloned and sequenced.The amplified fragments were assigned to their respective S-RNases by blast analysis.The S-genotypes of the eleven tested self-incompatible cultivars were identified as follows: Zaoshu Juju,Xinjiang Huanli,Lvjuju and Kuike Juju are all S22S28;Yuanxiang S16S15,Fuan Jianba S16S22,Jinmili S21S28,Lanzhou Ruaner SdS12,Chixianfeng ... |
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| Keywords: | science of non-timber Pyrus spp self-incompatibility S-allele S-genotype |
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