桑树轮纹病病原菌的分离鉴定及其生物学特性研究

2012年6～8月，通过对桑树轮纹病病原菌分离、田间致病性测定、病原菌形态观察，将该病原菌鉴定为桑膝节霉，并对此病病原菌的生物学特性进行了研究。结果表明，不同培养基配方、温度、酸碱度、碳源、氮源、光照对桑树轮纹病病原菌菌丝的生长有一定影响，菌丝生长的最适宜培养基是理查培养基，最适温度是25℃，最适pH值是7.0，最适碳源为葡萄糖，最适氮源为蛋白胨，光照对菌丝生长无显著性差异，菌丝的致死温度和时间分别为85℃和10 min。对该病病原菌生物学特性研究为云南首次报道，对桑树轮纹病的防治具有一定的参考价值。

Isolation and Biological Characteristics of Pathogenic Fungus Causing Ring Rot on Mulberry Leaves

From June to August , 2012, the pathogenic fungi of mulberry ring rot disease was isolated , its pathoge-nicity was determined in the feild , its morphology was observed , and this pathogenic fungus was identified as Gona-tophragmium mori.Study on the biological characteristics of the pathogenic fungus showed that medium , tempera-ture , pH, carbon source , nitrogen source and light had certain influence on mycelial growth , the optimal media for mycelial growth was Richard media , the optimum temperature for mycelial growth was 25℃and pH value was 7, the best carbon source and nitrogen source for mycelial grow are glucose and peptone , light had no significant effects on mycelial growth , the mycelial couldn ’ t survive above 85℃for 10min.This is the first report on the study of Gonato-phragmium mori in Yunnan , and could provide reference on mulberry ring rot disease prevention and control .