| 红松SRAP-PCR反应体系的优化 |
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| 引用本文： | 冯健,王骞春,于世河,杨鹤,李秀玲,吴树成.红松SRAP-PCR反应体系的优化[J].辽宁林业科技,2010(6):6-8. |
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| 作者姓名： | 冯健 王骞春 于世河 杨鹤 李秀玲 吴树成 |
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| 作者单位： | [1]辽宁省林业科学研究院,辽宁沈阳110032 [2]抚顺县前甸林场,辽宁抚顺113103 [3]抚顺县温道林场,辽宁抚顺113113 |
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| 基金项目： | 辽宁省科技厅攻关项目，辽宁省博士启动基金项目 |
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| 摘 要： | 通过单因素试验，对红松SRAP—PCR反应体系的主要影响因子（Taq DNA聚合酶、Mg^2＋、模板DNA、dNTPs、引物浓度）进行了优化筛选，得出红松SRAP—PCR反应的最佳体系：20μL反应体系中，模板90ng，Mg^2＋1．0mmol／L，dNTPs0．15mmol／L，Taq DNA聚合酶1．5 U，引物0．3～0．4μmol／L。
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| 关 键 词： | 红松 SRAP—PCR 反应体系 |
Optimization of the SRAP-PCR reaction systems of Pinus koraiensis |
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| FENG Jian,WANG Qian-chun,YU Shi-he,YANG He,LI Xiu-ling,WU Shu-cheng.Optimization of the SRAP-PCR reaction systems of Pinus koraiensis[J].Liaoning Forestry Science and Technology,2010(6):6-8. |
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| Authors: | FENG Jian WANG Qian-chun YU Shi-he YANG He LI Xiu-ling WU Shu-cheng |
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| Institution: | 1. Liaoning Academy of Forestry Sciences, Shenyang 110032, China; 2. Fushun County Qiandian Forest Farm, Fushun 113103, China; 3. Fushun County Wendao Forest Farm, Fushun 113113, China) |
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| Abstract: | Optimum selection were conducted on the major influenfial factors （Taq DNA polymerase, Mg^2＋, template DNA, dNTPs and concentration of primer） for the SRAP-PCR reaction systems of Pinus koraiensis by means of one-factor experiments, and the best SRAP-PCR reaction systems of Pinus koraiensis were worked out： in the 201aL reaction systems, there were 90 ng of template, 1.0 mmol·L^-1 of Mg^2＋, 0.15 mmol·L^-1 of dNTPs, 1.5 U ofTaq DNA polymerase and 0.3 - 0.4 μmol · L^-1 of primer. |
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| Keywords: | Pinus koraiensis SRAP-PCR re action systems |
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