白芨的离体培养

为快速生产大量优质白芨种苗,以白芨种子为外植体进行离体快繁研究。结果表明:MS基本培养基可促进白芨种子的快速无菌萌发,当6-BA的浓度为1.0 mg/L时可以促进幼苗的快速分裂生长,添加了2 mg/L的2,4-D的培养基可有效促进愈伤组织的形成。种子苗原球茎经切割后在MS+2 mg/L 2,4-D+0.5 mg/L6-BA的培养基上愈伤组织诱导率为85%,1.0 mg/L的6-BA和0.15 mg/L的NAA配比可以诱导丛生芽的生长和增殖,添加了70 g/L香蕉泥和0.5 mg/L NAA的1/2MS培养基可有效促进根的生长。无菌发芽的种子苗移栽率可以达90%,而试管苗移栽的成活率较低,但随离体培养时间的延长有所提高。

Culture in vitro of Bletilla striata

To propagate the high quality seedlings of Bletilla striata rapidly,the seeds were used as explants for in vitro culture.The results showed that,MS basic medium could promote the rapid aseptic germination of bletilla seeds.When the concentration of 6—BA was 1.0 mg/L could promote the rapid growth of seedlings.The medium added with 2 mg/L 2,4—D could effectively promote the formation of callus.The callus induction rate on the medium of MS+2 mg/L 2,4—D+0.5 mg/L 6—BA was 85%.1.0 mg/L 6—BA and 0.15 mg/L NAA could induce the rapid proliferation of clump buds.And the medium of 1/2MS added with 0.5 mg/L NAA and 70 g/L banana puree could effectively promote the root development.The transplant survival rate of aseptic seedling was 90%,whereas the survival rate of the plantlets was lower.