口蹄疫病毒3AB试剂盒的检测及比较

以重组口蹄疫病毒非结构蛋白3AB为检测抗原,研制了口蹄疫病毒非结构蛋白ELISA检测试剂盒,该试剂盒可用于判定被检动物是否感染口蹄疫病毒。重组口蹄疫病毒非结构蛋白3AB作为检测抗原包被酶标板反应孔,以辣根过氧化物酶标记的重组蛋白A/G作为第二抗体,建立了抗非结构蛋白抗体检测的方法。结果表明,在1 746份免疫猪血清中,3AB-ELISA对免疫猪血清的特异性为98.68%;在1 077份健康非免疫猪血清中,3AB-ELISA对健康非免疫猪血清的特异性为98.68%;在36份人工感染猪血清中,3AB-ELISA对阳性检出率为100%。而牛的特异性的各项指标和阳性检出率分别为94.04%,97.96%和100%。猪/牛口蹄疫病毒非结构蛋白酶联免疫吸附试验检测试剂盒与国外同类试剂盒比较,阳性检出率高出12.5个百分点。

Detection and comparison of diagnostic kits for FMDV-3AB

In order to judge if the detected animal is infected with foot-and-mouth disease virus(FMDV),a FMDV-NS 3AB ELISA diagnostic kit was developed by using the recombinant non-structural protein 3AB as a detecting antigen.The reaction microplate wells were coated with the detecting antigen based on the non-structural protein 3AB,the recombined protein A/G marked with horseradish peroxidase was used as the second antibody,and the anti-nonstructural protein antibody detecting method was established.The specificity of FMDV-NS 3AB ELISA diagnostic kit was 96.68% in 1 746 immune swine serums and 7.96% in 1 077 normal swineserums;its positive detection rate was 100% for 36 swine serums artificially infected with FMDV.And for bovine serums the above indexes were respectively 94.04%,97.96% and 100%.The positive detection rate of the enzyme-linked immunosorbent assay kit for FMDV-3AB of swine /bovine was 12.5 percentage points higher than that of the similar kits abroad.