| 禽源衣原体主要外膜蛋白基因的克隆与序列分析 |
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| 引用本文： | 王金福,张永霞,张似青,郁金观,顾剑新,孙维平.禽源衣原体主要外膜蛋白基因的克隆与序列分析[J].上海农业学报,2007,23(1):12-15. |
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| 作者姓名： | 王金福 张永霞 张似青 郁金观 顾剑新 孙维平 |
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| 作者单位： | 上海农林职业技术学院动物科学技术系 上海,201600 |
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| 基金项目： | 上海农林职业技术学院青年教师资金资助项目 |
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| 摘 要： | 根据GenBank中收录的鹦鹉热衣原体主要外膜蛋白(MOMP)基因序列设计合成1对引物,以禽源鹦鹉热衣原体基因组为模板,应用PCR扩增MOMP基因,将其克隆到pUCm-T载体上,并进行序列分析。结果显示,本株禽源鹦鹉热衣原体MOMP编码基因全长为1176 bp,与国外报道的火鸡鹦鹉热衣原体TT3株的MOMP基因同源性为91.9%,与鹦鹉鹦鹉热衣原体6BC株MOMP基因的同源性为83.1%。
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| 关 键 词： | 鹦鹉热衣原体 MOMP基因 序列分析 |
| 文章编号： | 1000-3924（2007）01-12-04 |
| 修稿时间： | 2006-01-242006-12-31 |
Cloning and sequence analysis of major outer membrane protein gene of avian chlamydia |
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| WANG Jin-fu,ZHANG Yong-xia,ZHANG Si-qing,YU Jin-guan,GU Jian-xin,SUN Wei-ping.Cloning and sequence analysis of major outer membrane protein gene of avian chlamydia[J].Acta Agriculturae Shanghai,2007,23(1):12-15. |
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| Authors: | WANG Jin-fu ZHANG Yong-xia ZHANG Si-qing YU Jin-guan GU Jian-xin SUN Wei-ping |
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| Institution: | Shanghai Vocational and Technological College of Agriculture and Forestry, Shanghai 201600, China |
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| Abstract: | According to the major outer membrane protein(MOMP) gene sequence of chlamydia of psittacosis recorded in GenBank and by using the genome of chlamydia of psittacosis isolated in Inner Mongolia as a template,a pair of primers were successifully designed to amplify MOMP gene by PCR.Then it was cloned into pUCm-T vector and sequenced.The results showed that the full length of the encoding gene of the MOMP gene of this strain was 1 176 bp and that the homology of MOMP gene was 91.9% between this strain and TT3 isolated from turkey and 83.1% between this strain and the 6BC strain from psittacosis chlamydia. |
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| Keywords: | Chlamydia of psittacosis MOMP gene Sequence analysis |
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