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| 优良八仙花品种(Hydrangea serrata‘Preziosa’)的离体培养与快速繁殖 | |
| 引用本文： | 殷丽青,胡永红,汤桂钧,黄卫昌,李世忠,王新其.优良八仙花品种(Hydrangea serrata‘Preziosa’)的离体培养与快速繁殖[J].上海农业学报,2010,26(1):38-41. |
| 作者姓名： | 殷丽青 胡永红 汤桂钧 黄卫昌 李世忠 王新其 |
| 作者单位： | 1. 上海市农业科学院林木果树研究所,上海,201106 2. 上海植物园,上海,200231 3. 上海市闵行区农业科学研究所,上海,201109 |
| 基金项目： | 上海市科委创新基金，上海市科技兴农重点攻关项目 |
| 摘 要： | 以八仙花当年生茎段为外植体，进行离体快速繁殖技术研究。结果表明：无菌芽诱导的适宜培养基为改良MS＋BA1．0mg／L＋NAA0．1mg／L＋CH0．5g／L十蔗糖30g／L＋琼脂粉6．0g／L，诱导率为97．2％；芽增殖的适宜培养基为改良MS＋BA1．0mg/L＋蔗糖30g/L＋琼脂粉6．0g/L，增殖系数达6．1；生根的适宜培养基为1／2MS＋IBA0．3mg／L＋蔗糖20g/L＋琼脂粉6．0g／L，试管苗在该培养基上根系生长良好，生根率达100％。 |
| 关 键 词： | 八仙花 组织培养 快速繁殖 观赏灌木 |
In vitro culture and rapid propagation of Hydrangea serrata 'Preziosa' |
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| YIN Li-qing,HU Yong-hong,TANG Gui-jun,HUANG Wei-chang,LI Shi-zhong,WANG Xin-qi.In vitro culture and rapid propagation of Hydrangea serrata ''Preziosa''[J].Acta Agriculturae Shanghai,2010,26(1):38-41. | |
| Authors: | YIN Li-qing HU Yong-hong TANG Gui-jun HUANG Wei-chang LI Shi-zhong WANG Xin-qi |
| Institution: | YIN Li-qing1,HU Yong-hong2,TANG Gui-jun3,HUANG Wei-chang2,LI Shi-zhong3,WANG Xin-qi1(1Forestry , Pomology Research Institute,SAAS,Shanghai 201106,China,2Shanghai Botanical Garden,Shanghai 200231,3Agricultural Research Institute of Minghang District,Shanghai 201109,China) |
| Abstract: | Current stem segments of Hydrangea serrata 'Preziosa' were used as explants and rapid in vitro propagation of Hydrangea serrata 'Preziosa' were studied.The results showed that the medium suitable for inducion of the germ-free buds was the improved MS+1.0 mg/L BA+0.1 mg/L NAA+ 0.5 g/L CH+30 g/L sugar+6.0 g/L agar,with the inductivity of buds being 97.2%;the medium suitable for multiplication of the clumpy buds was the improved MS+1.0 mg/L BA+30 g/L sugar+6.0 g/L agar,with the multiplication rate being 6.1;an... |
| Keywords: | Hydrangea serrata 'Preziosa' Tissue culture Rapid propagation Ornamental shrub |
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