| 利用PCR和限制性酶切技术鉴别3种鳗鱼 |
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| 引用本文： | 刘光明,史千玉,曹敏杰,苏国成,倪辉,蔡慧农.利用PCR和限制性酶切技术鉴别3种鳗鱼[J].厦门水产学院学报,2011(3):178-181. |
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| 作者姓名： | 刘光明 史千玉 曹敏杰 苏国成 倪辉 蔡慧农 |
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| 作者单位： | [1]集美大学生物工程学院,福建厦门361021 [2]福建省高校水产科学技术与食品安全重点实验室,福建厦门361121 |
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| 基金项目： | 农业部农业公益性行业科研专项项目（nyhyzx07-043）; 国家自然科学基金资助项目（20872049） |
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| 摘 要： | 根据日本鳗、欧洲鳗和美洲鳗3种鳗鱼的16S rRNA基因序列,设计特异性引物,进行16SrRNA基因的PCR扩增,然后对PCR产物进行ApaI酶切反应.结果显示,日本鳗和欧洲鳗的PCR产物均出现211 bp的特异性扩增条带,美洲鳗的PCR产物出现两条DNA条带;日本鳗的PCR产物经ApaI酶切产生大小为135 bp和76 bp的两条条带,而欧洲鳗的PCR产物经ApaI酶切没有变化.因此,利用该方法可鉴别出日本鳗、欧洲鳗和美洲鳗等3种鳗鱼.
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| 关 键 词： | 鳗鱼 16S rRNA PCR 限制性酶切 分子鉴别 |
Identification of Three Eel Species with PCR and Restriction Analysis |
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| Authors: | LIU Guang-ming SHI Qian-yu CAO Min-jie SU Guo-cheng NI Hui CAI Hui-nong |
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| Institution: | 1.School of Biotechnology Engineering,Jimei University,Xiamen 361021,China;2.Key Laboratory of Science andTechnology for Aquaculture and Food Safety(Jimei University),Fujian Province,Xiamen 361021,China) |
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| Abstract: | According to the sequences of 16S rRNA genes of three eels（Anguilla japonica,Anguilla anguilla,and Anguilla rostrata）,a pair of primers were designed for amplification of partial 16S rRNA genes.The PCR products were recycled and digested with restriction enzyme Apa I.Electrophoretic analysis of the PCR products from A.japonica and A.anguilla did not indicate any difference of one band in length of 211 bp,but the PCR products from A.rostrata had two bands.Since only partial 16S rRNA gene of A.japonica had the restriction site of Apa I,two fragments of 135 bp and 76 bp were presented only for the PCR products from A.japonica through Apa I,while the PCR products from A.anguilla were not digested with Apa I.PCR amplification and Apa I restriction analysis of partial 16S rRNA gene of A.japonica,A.anguilla and A.rostrata were suitable genetic markers to distinguish three eel species. |
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| Keywords: | eel 16S rRNA PCR restriction analysis molecular identification |
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