| MUG单管定量检测法对大肠埃希氏菌的检测 |
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| 引用本文： | 张小东,;鄢庆枇,;邹文政,;纪荣兴.MUG单管定量检测法对大肠埃希氏菌的检测[J].厦门水产学院学报,2009(4):339-344. |
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| 作者姓名： | 张小东 ;鄢庆枇 ;邹文政 ;纪荣兴 |
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| 作者单位： | [1]集美大学水产学院,福建厦门361021; [2]福建省高校水产科学技术与食品安全重点实验室,福建厦门361021 |
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| 基金项目： | 国家863计划项目（2007AA09Z115）;厦门市科技计划项目（3502Z20073019）;厦门市海洋与渔业局科技项目（200707） |
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| 摘 要： | 采用MUG单管定量检测法对实验室制备的菌悬液、天然海水、水产品进行定量检测,并与大肠埃希氏菌多管发酵法的检测结果进行比对分析.结果表明：MUG单管定量检测法与大肠埃希氏菌多管发酵法对菌悬液、天然海水、水产品的检测结果没有统计学差异;MUG单管定量检测法检测数据的精确性高于大肠埃希氏菌多管发酵法,而且更为快速、经济.因此,该法适用于海水和水产品中大肠埃希氏菌的定量检测.
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| 关 键 词： | 海水 水产品 大肠埃希氏菌 MUG单管定量检测法 大肠埃希氏菌多管发酵法 |
Detection of Escherichia coli by MUG Single Tube Quantitative Assay Method |
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| Institution: | ZHANG Xiao-dong, YAN Qing-pi, ZOU Wen-zheng, JI Rong-xing ( 1. Fisheries College, Jimei University, Xiamen 36102i, China; 2. Key Laboratory of Science and Technology for Aquaculture and Food Safety (Jimei University), Fujian Province, Xiamen 361021, China) |
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| Abstract: | MUG single tube quantitative assay method was introduced to Escherichia coil assay for bacterial suspensions, natural seawater, and fishery product samples. The detected data was analyzed and compared with multiple tube fermentation method. The results showed： no significant difference was found between the results of single tube quantitative assay method and multiple tube fermentation technique; single tube quantitative assay method showed better accurateness, precision, and reproducibility than multiple tube fermentation technique; single tube quantitative assay method was quicker and more economical. The results indicated that MUG single tube quantitative assay method was suitable for the E-coli enumeration in seawater and fishery products. |
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| Keywords: | seawater fishery product Escherichia coli MUG single tube quantitative assay method multiple tube fermentation technique for Escherichia coli |
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