褐绿色型与野生色型坛紫菜杂交产生嵌合体及其选育 |
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引用本文: | 王凤霞,谢潮添,陈昌生,纪德华,徐燕,史修周,梁艳,张元.褐绿色型与野生色型坛紫菜杂交产生嵌合体及其选育[J].厦门水产学院学报,2010(1):1-6. |
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作者姓名: | 王凤霞 谢潮添 陈昌生 纪德华 徐燕 史修周 梁艳 张元 |
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作者单位: | [1]集美大学水产学院,福建厦门361021 [2]福建省高校水产科学技术与食品安全重点实验室,福建厦门361021 |
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基金项目: | [基金项目]国家科技支撑项目(2007BAD07803);国家自然科学基金资助项目(40676077);国家海洋863资助项目(2006AA10A413);福建省重大科技平台建设项目(2007N2011) |
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摘 要: | 以野生型坛紫菜为父本,人工选育的褐绿色型坛紫菜为母本进行杂交实验,获得大量的单色体和嵌合体,在其子一代中随机抽取1147株藻体进行统计分析,并对生长性状好的藻体进行选育培养,结果显示:1)嵌合体色泽:主要为野生色+褐绿色、野生色+褐绿色+野生色、褐绿色+野生色+褐绿色;2)嵌合体的比例:单一色型藻体占14.5%,2种色泽相嵌的嵌合体占73.9%,3种色泽的嵌合体占10.6%,4种色泽的嵌合体占1.1%;3)嵌合方式:点状、线型、“V”字型、“w”字型和小叶片型等;4)通过营养细胞酶解和单性生殖所获得的单色藻体c,经培养后F1和F2代遗传性状比较稳定,有望筛选成为新的品系.
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关 键 词: | 坛紫菜 杂交 嵌合体 选育 |
Study on Chimeras and Breeding from Hybridization of the Breen-type and the Wild-type Porphyra haitanensis |
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Authors: | WANG Feng-xia XIE Chao-tian CHEN Chang-sheng JI De-hua XU Yan SHI Xiu-zhou LIANG YahI ZHANG Yuan |
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Institution: | 1. Fisheries College, Jimei University, Xiamen 361021, China; 2. Key Laboratory of Science and Technology for Aquaeuhure and Food Safety (Jimei University) , Fujian Province, Xiamen 361021, China) |
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Abstract: | The breen-type gained from artificial breeding and the wild-type Porphyra haitanensis were re- spectively used as female and male parent for hybridization, and lots of monochromatic thalli and chimeras were obtained. Further more, 1 147 strains of F1 seedlings were taken as examples and thalli with good growth trait were selected and cultured. The results indicated: 1 ) Colors of the chimera: wild and breen; wild, breen and wild; breen, wild and breen; 2) Percentage of chimeras: The chimeras with mono color accounted for 14. 5%, with two colors accounted for 73.9 %, those with three colors accounted for 10. 6 % and those with four colors I. 1% ; 3 ) Chimeric patterns: spot type, line type, "V" type, "W" type, blade type; 4) Monochromatic thalli c strain was obtained from enzymolysis and parthenogenesis, and its heritable traits, including F1 and F2, were fairly stable. Monochromatic thalli c strain had ordinary economic traits and would be selected to be a new Porphyra strain. |
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Keywords: | Porphyra haitanensis hybridization chimera selection |
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