| 左伏3号山楂的组织培养和常温离体保存研究 |
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| 引用本文： | 王秋竹,林丽华,董文轩.左伏3号山楂的组织培养和常温离体保存研究[J].农村实用技术与信息,2009(5):48-50. |
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| 作者姓名： | 王秋竹 林丽华 董文轩 |
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| 作者单位： | [1]沈阳农业大学园艺学院,辽宁沈阳110161 [2]吉林农业科技学院,吉林132101 |
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| 基金项目： | 国家科技基础条件平台项目（2005DKA21002-29） |
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| 摘 要： | 以伏山楂品种＂左伏3号＂为试验材料,进行组织培养和常温离体保存研究。结果表明：左伏3号启动培养的最佳培养基是MS＋6-BA1.0 mg.L-1＋IAA0.08 mg.L-1,成活率达到93.3%;继代培养的最佳培养基是MS＋6-BA0.8 mg.L-1＋NAA0.01 mg.L-1,繁殖系数达到5.5;生根培养在1/2MS＋NAA0.5 mg.L-1上生根率（31.7%）最高,移栽可成活。0.4～2.0 mg.L-1的PP333不适宜左伏3号的常温离体保存,而CCC 40 mg.L-1和ABA2.0 mg.L-1比较适合。
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| 关 键 词： | 山楂 组织培养 生长抑制剂 离体保存 |
Hawthorn V left on The 3rd and The Normal Temperature of The Tissue Culture Studies in Vitro Preservation |
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| Institution: | WANG Qiu -zhu, LIN Li - hua, DONG Wen - xuan (1. University of Horticulture, Shenyang Agricultural University, Shenyang Haoning 110161, china; 2. Jiiin Academy of Agricultural Science and Technology, Jilin 132101, China) |
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| Abstract: | Hawthorn varieties V ＂left on the 3rd V＂ as the test material, and at room temperature for tissue culture studies in vitro preservation. The results showed that： V 3, left to start training is the best medium MS ＋ 6 - BA 1.0 mg o L - 1 ＋ IAA 0.08 mg o L - 1, the survival rate reached 93.3% ; Subculture is the best medium MS ＋ 6 - BA 0.8 mg o L - 1 ＋ NAA 0.01 mg o L - 1, propagation coefficient of 5.5 ; roots were cultured in 1/2MS ＋ NAA 0.5 mg o L - 1 on the rooting rate （31,7%） the highest, transplanting can be survived. 0.4 -2.0 mg o L - 1 of PP333 inappropriate left at room temperature of V 3 in vitro preservation, and CCC 40 mg o L - 1 and ABA 2.0 mg o L - 1 is more suitable. |
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| Keywords: | Hawthorn tissue culture growth inhibitor vitro conservation |
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