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| 沙冬青根瘤菌表型特征和16SrDNAPCR-RFLP分析 | |
| 引用本文： | 毕江涛,谢瑞梅,位秀丽,贺达汉,韦革宏.沙冬青根瘤菌表型特征和16SrDNAPCR-RFLP分析[J].农业科学研究,2009,30(3):14-20,27. |
| 作者姓名： | 毕江涛 谢瑞梅 位秀丽 贺达汉 韦革宏 |
| 作者单位： | 1. 宁夏大学农学院,宁夏银川,750021;西北农林科技大学微生物研究中心,陕西杨凌,712100;宁夏农业技术推广总站,宁夏银川,750001 2. 西北农林科技大学微生物研究中心,陕西杨凌,712100 3. 宁夏大学农学院,宁夏银川,750021 |
| 基金项目： | 宁夏人事厅出国留学人员科研活动资助项目，西部农林高校博士生访学研究基地项目 |
| 摘 要： | 应用表型数值分类和16S rDNA PCR-RFLP 分析方法,对分离自宁夏和内蒙古部分地区的沙冬青根瘤菌进行表型和遗传多样性研究.通过分离纯化获得44株供试根瘤菌,选取37株未知菌和11株参比菌株进行唯一碳氮源利用、抗生素和染料抗性、耐盐性、初始pH生长、生长温度范围和脲酶共94项生理生化性状测定,以及16S rDNA 基因扩增和扩增产物的限制性酶切分析.结果表明,沙冬青根瘤菌在碳源利用、抗生素敏感性和染料抗性方面存在差异;对15项所选氨基酸氮源均能利用,无明显差异;具有较强的耐盐碱和耐高温能力.经过数值分类和16S rDNA PCR-RFLP比较分析,测试菌株具有丰富的表型和遗传多样性.测试菌株和参比菌株在80%的相似水平上产生2个表观群,表观群I和表观群II在鉴别特征上存在差异.数值分类中聚群的根瘤菌菌株在16S rDNA PCR-RFLP分析中也聚群,2种分析方法有基本的一致性. |
| 关 键 词： | 沙冬青 根瘤菌 表型多样性 多聚酶链式反应 限制性片段长度多态性 |
Analysis of phenotypic diversity of rhizobia isolated from Ammopiptanthus mongolicus and 16S rDNA PCR-RFLP |
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| Bi Jiangtao,Xie Ruimei,Wei Xiuli,He Dahan,Wei Gehong.Analysis of phenotypic diversity of rhizobia isolated from Ammopiptanthus mongolicus and 16S rDNA PCR-RFLP[J].Journal of Agricultural Sciences,2009,30(3):14-20,27. | |
| Authors: | Bi Jiangtao Xie Ruimei Wei Xiuli He Dahan Wei Gehong |
| Institution: | Bi Jiangtao1,2,3,Xie Ruimei2,Wei Xiuli2,He Dahan1,Wei Gehong2(1.School of Agriculture,Ningxia University,Yinchuan 750012,China,2.Research Center for Microbe,Northwest University of A&F,Yangling 712100,3.Ningxia Service Station for Extension of Agricultural Techniques,Yinchuan 750001,China) |
| Abstract: | Phenotypic and genetic diversities of rhizobia isolated from Ammopiptanthus mongolicus growing in parts area of Ningxia and Inner Mongolia were analyzed with the methods of numerical taxonomy and 16S rDNA PCR-RFLP.44 rhizobium strains were obtained through isolation and purification from root nodules,in which 37 unidentified tested strains and 11 reference strains were selected for 94 physiological and biological testing items such as utilization of sole carbon and sole nitrogen sources,antibiotics and dyes... |
| Keywords: | Ammopiptanthus mongolicus rhizobia phenotypic diversity polymerase chain reaction restriction fragment length polymorphism |
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