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| 紫薇叶片DNA的提取及AFLP反应体系的建立 | |
| 引用本文： | 王献,柴永生,职永普.紫薇叶片DNA的提取及AFLP反应体系的建立[J].河南农业大学学报,2004,38(2):189-192,230. |
| 作者姓名： | 王献 柴永生 职永普 |
| 作者单位： | 1. 河南农业大学林学园艺学院,河南,郑州,450002 2. 北京林业大学资源与环境学院,北京,100083 3. 河南省获嘉县国土资源局,河南,获嘉,453800 |
| 基金项目： | 河南省科技攻关项目(0124050022) |
| 摘 要： | 以紫薇叶片为材料，利用改进的酚一氯仿法，提取到了高质量的紫薇叶片DNA，并建立了20个紫薇品种和南紫薇的AFLP银染反应体系，首次在紫薇DNA提取、酶切连接、预扩增、选择性扩增等实验过程取得了成功，得到了清晰的紫薇品种的AFLP指纹图谱，为紫薇基因库的建立、优良品种选育以及亲缘关系的系列研究奠定了理论基础． |
| 关 键 词： | 紫薇 叶片DNA 提取 AFLP 反应体系 酚-氯仿法 基因库 指纹图谱 |
| 文章编号： | 1000-2340(2004)02-0189-04 |
Extraction of DNA and construction of AFLP fingerprinting in leaves of Lagerstromi indica |
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| WANG Xian,CHAI Yong-sheng,ZHI Yong-pu.Extraction of DNA and construction of AFLP fingerprinting in leaves of Lagerstromi indica[J].Journal of Henan Agricultural University,2004,38(2):189-192,230. | |
| Authors: | WANG Xian CHAI Yong-sheng ZHI Yong-pu |
| Institution: | WANG Xian~1,CHAI Yong-sheng~2,ZHI Yong-pu~3 |
| Abstract: | The study dealt with extraction of DNA with phenol-cholorofore method from L.indica leaves and established AFLP fingerprinting in 20 L.indica cultures and L.subcostata. indica Clear and high resolution DNA fingerprints were obtained,which means success in DNA extraction, restriction_ligase reaction, pre_amplification and staining techniques. The aim of this paper is to lay a foundation for the establishment of gene pool, selection and breeding of eminent cultivars and genetic relationship in L. indica. |
| Keywords: | L indica extraction of DNA AFLP fingerprints |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |