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玉米Hd6-like基因植物表达载体的构建及其功能分析
引用本文:王新涛,李思远,吴连成,库丽霞,常丽丽,杨爽,陈彦惠.玉米Hd6-like基因植物表达载体的构建及其功能分析[J].河南农业大学学报,2009,43(1).
作者姓名:王新涛  李思远  吴连成  库丽霞  常丽丽  杨爽  陈彦惠
作者单位:河南农业大学农学院,河南,郑州,450002
基金项目:国家高技术研究发展计划(863计划) 
摘    要:利用已克隆的玉米Hd 6-like基因构建植物过量表达栽体PBI-Hd 6,将Hd 6-like插入带有启动子CaMV 35 S和终止子nos的PBI 121中间载体中,经过酶切鉴定表明,目的基因已正确地插入该栽体中.利用根癌农杆菌侵染花序法侵染拟南芥,将Hd 6-like基因转入拟南芥,获得转基因Hd 6-ox植株,经PCR检测表明,该基因已整合到拟南芥的基因组中.转基因Hd 6-ox植株的花期推迟了24 d左右.

关 键 词:玉米  Hd6-like基因  表达载体构建

Construction of plant expression vector of Hd 6-like gene from maize and its function analysis
WANG Xin-tao,LI Si-yuan,WU Lian-cheng,KU Li-xia,CHANG LI-li,YANG Shuang,CHEN Yan-hui.Construction of plant expression vector of Hd 6-like gene from maize and its function analysis[J].Journal of Henan Agricultural University,2009,43(1).
Authors:WANG Xin-tao  LI Si-yuan  WU Lian-cheng  KU Li-xia  CHANG LI-li  YANG Shuang  CHEN Yan-hui
Institution:College of Agronomy;Henan Agricultural University;Zhengzhou 450002;China
Abstract:By inserting Hd 6-like in vector PBI 121 with promoter CaMV 35 S and nos terminator,a plant overexpression vector of Hd 6-like gene from maize was constructed successfully,then it was transformed into Arabidopsis with floral dip method,and got the transformant plant Hd 6-ox.The results of PCR showed that the gene was integrated into Arabidopsis genome.Hd 6-ox plants flowered later than wild type about 24 d under LD condition.
Keywords:maize  Hd 6-like gene  construction of expression vector  
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