| 包被LPS抗原检测鸡源鲍氏志贺氏菌抗体的间接ELISA方法的建立 |
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| 引用本文： | 邓久虎,许兰菊,杨永珍,张丹鹤,苗银萍,李炜.包被LPS抗原检测鸡源鲍氏志贺氏菌抗体的间接ELISA方法的建立[J].河南农业大学学报,2011,45(1):91-96. |
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| 作者姓名： | 邓久虎 许兰菊 杨永珍 张丹鹤 苗银萍 李炜 |
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| 作者单位： | 河南农业大学牧医工程学院,河南,郑州,450002 |
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| 基金项目： | 河南省重点科技攻关项目 |
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| 摘 要： | 以鸡源鲍氏志贺氏茵菌体提取的脂多糖(LPS)成分作为检测抗原,以辣根过氧化物酶标记的免抗鸡IgG抗体为第二抗体,以TMB为显色剂,成功建立了检测鸡源鲍氏志贺氏茵抗体的间接ELISA方法.通过对间接ELISA反应条件的一系列研究,确定了各种最适工作条件:鸡源鲍氏志贺氏茵LPS抗原最适包被质量浓度为5mg·L-1,最适包被...
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| 关 键 词： | 鸡源鲍氏志贺氏菌 LPS 抗体 间接ELISA |
The establishment of the indirect ELISA method for the detecting of Shigella boydii antibody from chicken by using LPS as a coating antigen |
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| DENG Jiu-hu,XU Lan-ju,YANG Yong-zhen,ZHANG Dan-he,MIAO Yin-ping,LI Wei.The establishment of the indirect ELISA method for the detecting of Shigella boydii antibody from chicken by using LPS as a coating antigen[J].Journal of Henan Agricultural University,2011,45(1):91-96. |
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| Authors: | DENG Jiu-hu XU Lan-ju YANG Yong-zhen ZHANG Dan-he MIAO Yin-ping LI Wei |
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| Institution: | DENG Jiu-hu,XU Lan-ju,YANG Yong-zhen,ZHANG Dan-he,MIAO Yin-ping,LI Wei(Engineering College of Animal Husbandry and Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China) |
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| Abstract: | |
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| Keywords: | LPS |
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