| 禽流感病毒H5,H7,H9亚型多重RT-PCR检测方法的建立 |
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| 引用本文： | 胡建和,赵坤,杭柏林,荆汝顶,王丽荣,王三虎,刘兴友.禽流感病毒H5,H7,H9亚型多重RT-PCR检测方法的建立[J].河南农业大学学报,2009,43(2). |
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| 作者姓名： | 胡建和 赵坤 杭柏林 荆汝顶 王丽荣 王三虎 刘兴友 |
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| 作者单位： | 1. 河南科技学院动物科学学院,河南,新乡,453003
2. 新乡市畜牧兽医工作站,河南,新乡,453003 |
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| 基金项目： | 河南省高等学校杰出科研人才创新工程项目 |
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| 摘 要： | 依据GenBank中亚洲流行株禽流感病毒(AIV)H5,H7,H9亚型血凝素基因及M基因序列保守区域设计特异性引物,利用多重反转录聚合酶链式反应技术建立了AIV及H5,H7,H9亚型的检测方法.该方法能一次性检测出AIV保守区域M基因的同时,直接区分H5,H7,H9亚型.对新城疲病毒、传染性法氏囊病毒、传染性支气管炎病毒、传染性喉气管炎病毒等特异性检测均为阴性.样品经100倍稀释后仍能检出.
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| 关 键 词： | 禽流感病毒 血凝素 多重RT-PCR 检测 |
Construction of multiplex RT-PCR for detection of H5,H7 and H9 subtypes of avian influenza virus |
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| HU Jian-he,ZHAO Kun,HANG Bo-lin,JING Ru-ding,WANG Li-rong,WANG San-hu,LIU Xing-you.Construction of multiplex RT-PCR for detection of H5,H7 and H9 subtypes of avian influenza virus[J].Journal of Henan Agricultural University,2009,43(2). |
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| Authors: | HU Jian-he ZHAO Kun HANG Bo-lin JING Ru-ding WANG Li-rong WANG San-hu LIU Xing-you |
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| Institution: | 1.School of Animal Science;Henan Institute of Science and Technology;Xinxiang 453003;China;2.Animal Husbandry and Veterinary Bureau of Xinxiang;China |
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| Abstract: | A multiplex RT-PCR was constructed to detect H5,H7,H9 subtypes of avian influenza virus.Four sets of specific oligonucleotide primers were designed for detection and differentiation of H5,H7 and H9 subtypes of avian influenza virus,which was based on sequences of hemagglutinin gene and matrix gene of avian influenza virus from GenBank.This method could detect matrix gene of AIV,and simultaneously identify H5,H7 and H9 subtypes.Detections of newcastle disease virus,infectious bursal disease virus,infectious ... |
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| Keywords: | avian influenza virus hemagglutinin multiplex RT-PCR detection |
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