| 玉米组氨酸三聚体核苷结合蛋白基因ZmHINT1的克隆及其原核表达载体构建 |
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| 引用本文： | 吴刘记,吴连成,祖小峰,王平安,陈彦惠.玉米组氨酸三聚体核苷结合蛋白基因ZmHINT1的克隆及其原核表达载体构建[J].河南农业大学学报,2011,45(3):267-270,279. |
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| 作者姓名： | 吴刘记 吴连成 祖小峰 王平安 陈彦惠 |
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| 作者单位： | 河南农业大学农学院,河南郑州,450002 |
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| 基金项目： | 国家973课题(2009CB118400); 河南省科技攻关计划项目(102102110171); 第47批中国博士后科学基金(20100470993) |
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| 摘 要： | 通过RT - PCR方法和基因克隆技术对玉米黄早4HINT 1基因进行了分子克隆和序列分析.结果表明,黄早4 HINT 1 cDNA全长为729 bp,氨基酸序列与动物HINT 1有较高的相似度,并且包含HITN1蛋白家族保守的活性位点区域.同时,为进一步研究玉米HINT1基因的理化性质及生物功能,利用PET - 28...
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| 关 键 词： | 玉米 组氨酸三聚体核苷结合蛋白 分子克隆 原核表达 |
Cloning and construction of prokaryotic expression vector of histidine triad nucleotide binding protein from maize Huangzao 4 |
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| WU Liu-ji,WU Lian-cheng,ZU Xiao-feng,WANG Ping-an,CHEN Yan-hui.Cloning and construction of prokaryotic expression vector of histidine triad nucleotide binding protein from maize Huangzao 4[J].Journal of Henan Agricultural University,2011,45(3):267-270,279. |
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| Authors: | WU Liu-ji WU Lian-cheng ZU Xiao-feng WANG Ping-an CHEN Yan-hui |
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| Institution: | WU Liu-ji,WU Lian-cheng,ZU Xiao-feng,WANG Ping-an,CHEN Yan-hui(College of Agronomy,Henan Agricultural University,Zhengzhou 450002,China) |
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| Abstract: | Molecular cloning and sequence analysis of Huangzao 4 HINT 1(Histidine triad nucleotide binding protein 1) were carried out based on RT-PCR method and gene cloning technique.The full length cDNA of Huangzao 4 HINT 1 is 729 bp,holding higher similarity with animal HINT 1 homologues and the conserved domains.PET-28a was used to construct the prokaryotic expression vector of Huangzao 4 HINT 1 for the further research on physiological and biochemical characterization of maize HINT 1. |
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| Keywords: | maize histidine triad nucleotide binding protein molecular cloning prokaryotic expre-ssion |
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