盐胁迫对南方泡桐基因表达的影响

为阐明泡桐多倍体的耐盐分子机制,以南方泡桐二倍体及对应的四倍体为试验材料,以白花泡桐基因组为参考序列,利用RNA-Seq测序技术,研究了盐处理前后南方泡桐四倍体和对应二倍体的基因表达变化。通过比对分析,共鉴定出与盐胁迫相关的差异表达基因2 940个。对这些差异基因进行GO功能分类,结果显示差异基因共参与了39个分类,集中在细胞、结合、催化等分类功能区的数量最多。KEGG代谢通路分析发现,这些差异表达基因主要参与了Plant hormone signal transduction,Carbon metabolism,Biosynthesis of amino acid等,其中编码MYB、WRKY、bZIP、ATPase等的基因差异表达显著。表明这些基因可能是潜在的盐胁迫响应基因。采用qRTPCR技术验证了随机挑选的9个差异表达基因,结果与转录组测序数据趋势一致。

Effect of salt stress on gene expressions of different genotypes of Paulownia australis

To elucidate the molecular mechanism of polyploidy Paulownia response to salt stress,RNA-Seq technology was used to research changes of genes in response to salinity tolerance in tetraploid Paulownia australis and its diploid with Paulownia reference genome sequences.A total of 2 940 differentially expressed genes related to salt stress were identified in four libraries.GO function classification showed that the differentially expressed genes were involved in 39 categories,and most of them involved in cells,binding,catalysis and other classifications.KEGG pathway analysis indicated that these differentially expressed genes were mainly involved in the synthesis of plant hormone signal transduction,carbon metabolism,and biosynthesis of amino acid.Among these differentially expressed genes,MYB,WRKY,and bZIP,ATPase were differentially expressered,suggesting that these genes may be potential genes of salt response.In addition,quantitative real-time PCR(qRT-PCR)technology was used to validate the expression data measured by RNA-seq for nine randomly selected differentially genes.These results suggested that the RNA-seq dataset was dependable.