山核桃FLOWERING LOCUST同源基因的克隆与序列分析

根据不同植物FLOWERING LOCUST（FT）同源基因序列的保守区，设计合成1对长度为18bp的PCR简并引物，以山核桃基因组DNA为模板，采用PCR方法扩增出长度为152bp的DNA片段，克隆到pMD19-T载体上。测序及序列分析结果表明，扩增所获得的片段序列为FT基因第四外显子部分序列，不含内含子，推测共编码50个氨基酸；其序列在GenBank中注册号为FJ858260．1，同源性比对结果表明，其氨基酸序列与其他植物FT基因的同源性高达88％～96％。

Cloning and Sequence Analysis of the FlOWERING LOCUS T Homologous Genes from Carya cathayensis

A pair of primers with 18 bp in length were designed according to a conserved sequence ot the homologous gene of different plant species FLOWERING LOCUS T（FT）, Taking the genomic DNA of Carya cathayenzis as template, a 152 bp long DNA fragment was amplified by polymerase chain reaction（ PCR）, And the fragment was cloned into a pMD19-T vector and then sequenced. The sequence analysis indicated that the deduced amino acid sequence of the fragment was part intron of the forth exon in the fragment with FT homologous genes encoding 50 amino acids, which was registered in GenBank with the accession number FJ858260.1. The homology test result showed that the deduced amino acid sequence of the fragment was 88% -96% homologous genes with FLOWERING LOCUS T （FT） of other plant species.