木瓜硬枝扦插繁殖技术与生根关联酶活性研究

以1年生曹州木瓜实生苗苗干为试材，采用L9（3^4）正交试验，研究木瓜扦插繁殖技术，测定生根过程中POD，PPO和IAAO3种酶活性的变化．结果表明：所有处理组合中，处理4（珍珠岩＋泥炭，IBA0．5mg／g处理30min）的组合方式最优，生根率可达66％；木瓜硬枝扦插生根过程可分为愈伤形成期、不定根诱导期和不定根伸长期3个阶段．3种生根关联酶活性变化呈现规律性，不同处理间和各阶段3种酶活性差异均显著．

Studies on Hardwood Cuttage Propagation Technique and the Activities of Related Oxidases of Carica papaya

The propagation technology and enzyme activities of indoleacetic acoxidase(IAAO),polyphenol oxidase(PPO) and peroxidase(POD) of Carica papaya were studied by orthogonal experiments design of L9(34) and by taking cuttings from one-year-old stems as experimental materials.The experiment results showed that rooting process by hard wood cuttings could be divided into three stages,i.e.,formation of callus,induction of adventitious roots and elongation of the adventitious roots.The treatment combination No.4(perlite peat,IBA 0.5mg/g,treatment for 30 min) was the best,whose rooting rate reached 66%,higher than other treatment combinations.It showed a rule for the activity change of three rooting related enzymes,there were significant differences in the activity of the three enzymes among the varied treatments and different stages.