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松针褐斑病菌毒素对紫茎泽兰伤害的初步研究
引用本文:王晓波,杨斌,付惠,鲁绍琴.松针褐斑病菌毒素对紫茎泽兰伤害的初步研究[J].西南林学院学报,2006,26(2):44-47.
作者姓名:王晓波  杨斌  付惠  鲁绍琴
作者单位:西南林学院,资源学院,云南,昆明,650224
基金项目:云南省青年基金资助项目(2003302)
摘    要:研究了松针褐斑病菌毒素对紫茎泽兰不同部位的伤害以及不同毒素粗提液浓度对叶片的伤害效果,进行了叶片愈伤组织的培养,在此基础上探讨了毒素对紫茎泽兰叶片和愈伤组织过氧化氢酶的影响.结果表明:紫茎泽兰叶片对该毒素最敏感,根对毒素只有轻微的反应;毒素粗提液稀释2倍,仍然对紫茎泽兰叶片有明显的伤害作用;以MS 6 mL(1 mg/mL)2,4-D培养紫茎泽兰叶片分化良好,愈伤组织生长速度快;毒素处理后叶片和愈伤组织CAT活性在6 h达到最高,呈现先升后降的趋势.

关 键 词:松针褐斑病菌  毒素  紫茎泽兰  愈伤组织  过氧化氢酶
文章编号:1003-7179(2006)-02-0044-04
收稿时间:2005-11-15
修稿时间:2005-11-15

A Preliminary Study on Damage of the Toxin Produced by Brown Spot Needle Blight Fungus to Eupatorium adenophorum
WANG Xiao-bo,YANG Bin,FU Hui,LU Shao-qin.A Preliminary Study on Damage of the Toxin Produced by Brown Spot Needle Blight Fungus to Eupatorium adenophorum[J].Journal of Southwest Forestry College,2006,26(2):44-47.
Authors:WANG Xiao-bo  YANG Bin  FU Hui  LU Shao-qin
Institution:Faculty of Resources, Southwest Forestry College, Kunming Yurman 650224, China
Abstract:The damage to Eupatorium adenophorum on different organs by toxin produced by brown spot needle blight fungus and the damage effect to the leaves of Eupatorium adenophorum by crude toxin extract of brown spot needle blight fungus with varied concentrations were studied.Bioassay methods was introduced to study the poisonous activity of the toxin to catalase of the callus and leaves of Eupatorium adenophorum by tissue culture.The results indicated that leaves of the weed were the most sensitive organ of the weed to the toxin,which could be significantly damaged by doubly diluted crude extract.The root was the least sensitive.Callus of Eupatorium adenophorum could differentiate and grow well in MS medium(with 6 mL of 2,4-D 1 mg /mL).Treated by toxin,the CAT activity of leaves and callus of Eupatorium adenophorum could rise to the uppermost within 6 h and then declined.
Keywords:Brown spot needle blight fungus  toxin  Eupatorium adenophorum  callus  catalase
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