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油茶花粉生活力检测方法比较
引用本文:袁德义,谭晓风,邹锋,崔明杰,梁玉琴.油茶花粉生活力检测方法比较[J].西南林学院学报,2009,29(4):10-12.
作者姓名:袁德义  谭晓风  邹锋  崔明杰  梁玉琴
作者单位:中南林业科技大学,国家林业局经济林育种与栽培重点实验室,湖南,长沙,410004
基金项目:国家"十一五"科技支撑计划,国家林业公益性行业科研专项(200804044)基金 
摘    要:以7个油茶种的花粉为试材,采用TTC法、无机酸法、离体萌发法、活体萌发法4种方法对其花粉生活力进行测定。结果表明:TTC法适合山茶属植物花粉生活力的检测,而无机酸法不适合;活体萌发法检测油茶花粉生活力不准确,但能有效表述花粉萌发力;离体萌发法能有效准确地测定油茶花粉生活力,适合山茶属植物花粉生活力的检测,是最为理想的快速测定法。

关 键 词:油茶  花粉活力  检测方法

Comparative Study on Pollen Viability DetectionMethods for Camellia oleifera
YUAN De-yi,TAN Xiao-feng,ZOU Feng,CUI Ming-jie,LIANG Yu-qin.Comparative Study on Pollen Viability DetectionMethods for Camellia oleifera[J].Journal of Southwest Forestry College,2009,29(4):10-12.
Authors:YUAN De-yi  TAN Xiao-feng  ZOU Feng  CUI Ming-jie  LIANG Yu-qin
Institution:( Key Lab of Non-timber Forest Tree Species Breeding and Cultivation, SFA, South Central University of Forestry Science and Technology, Changsha Hunan 410004,China)
Abstract:The pollen vitality of seven species in genus Camellia were determined by four different methods i. e. , triphenyl tetrazolium chloride (TI'C), mineral acid, in vitro and in vivo germination. The results indicated that ITC method, but not the mineral acid method, was suitable to detect the pollen viability of Camellia genus, The detect result obtained from in vivo germination method was not so accurate, but it could be applied to describe the pollen vigor more effectively. It was showed that the in vitro germination method was the best and the most ideal way to determine the pollen viability for species in Camellia genus.
Keywords:Camellia oleifera  pollen viability  detection method
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