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湿地松丛生芽增殖的研究
引用本文:何月秋,叶建仁,池树友.湿地松丛生芽增殖的研究[J].西南林学院学报,2005,25(3):14-17.
作者姓名:何月秋  叶建仁  池树友
作者单位:南京林业大学,森林资源与环境学院,江苏,南京,210037
基金项目:江苏省高等学校研究生创新计划.
摘    要:以湿地松无菌苗顶芽为外植体,诱导湿地松丛生芽的形成,诱导率可达100%.研究激素水平、基本培养基、继代周期、蔗糖浓度对湿地松丛生芽增殖的影响,结果表明:当6-BA为1mg/L,NAA为O.03mg/L时,最高增殖率可达85.71%,最高增值倍数为4.11;在5种基本培养基DCR,WPM,1/2MS,1/2GD,P6中,DCR比较适合丛生芽的增殖和生长;当培养基中蔗糖浓度为4%时,能够形成健壮的丛生芽;每隔20天继代1次,既能保持外植体的幼嫩状态.也能提高丛生芽的生长速度.

关 键 词:湿地松  组织培养  丛生芽  增殖
文章编号:1003-7179(2005)03-0014-04
收稿时间:2005-06-09
修稿时间:2005-06-09

Studies on Proliferation of Axillary Buds of Pinus elliottii
HE Yue-qiu,YE Jian-ren,CHI Shu-you.Studies on Proliferation of Axillary Buds of Pinus elliottii[J].Journal of Southwest Forestry College,2005,25(3):14-17.
Authors:HE Yue-qiu  YE Jian-ren  CHI Shu-you
Institution:College of Forest Resources and Environment, Nanjing Forestry University, Nanjing Jiangsu 210037, China
Abstract:Axillary buds of Pinus elliottii were induced when the apical buds were applied as explants, and the induction rate could reach 100%. The influences of plant growth regulators, basal medium, subculture interval, sucrose concentration on proliferation of axillary buds were experimented. It was showed by the results that the highest induction rate of axillary buds was up to 85. 71% on DCR medium supplemented with 1 mg/L 6 - BA and 0.03 mg/L NAA, the highest proliferation multiple was 4.11. Among 5 kinds of basal media tested, i.e., DCR, WPM, 1/2 MS, 1/2 GD and Pe, DCR was the best to the proliferation and growth of axillary buds. The DCR medium supplemented with 40 g/L of sucrose was suitable for proliferating healthy axillary buds. It was indicated that the best subculture interval was 20 days, which was good not only for keeping the explants in a tender state but also for speeding up the growth of the induced axillarv buds.
Keywords:Pinus elliottii  tissue culture  axillary buds  proliferation
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