| 葡萄SVP类MADS-box基因的克隆及表达分析 |
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| 引用本文： | 杨堃,张朝红,李树秀,张旭彤,王跃进.葡萄SVP类MADS-box基因的克隆及表达分析[J].西北林学院学报,2012,27(4):117-123. |
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| 作者姓名： | 杨堃 张朝红 李树秀 张旭彤 王跃进 |
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| 作者单位： | 西北农林科技大学园艺学院农业部西北地区园艺作物生物学与种质创制重点实验室旱区作物逆境生物学国家重点实验室,陕西杨陵,712100 |
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| 基金项目： | 国家自然科学基金项目(31171926);国家现代农业产业技术体系建设专项基金(CARS-30-yz-7);公益性行业(农业)科研专项(200903044) |
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| 摘 要： | 从无核白葡萄(Vitis vinifera cv.Thompson seedless)中克隆获得一个胚珠发育相关MADS-box基因。该基因cDNA全长1 248bp,ORF为729bp,编码一个含有243个氨基酸的蛋白。氨基酸多序列比对和系统发育分析显示,该基因属于SVP类MADS-box基因。采用半定量技术进行表达分析表明,该基因在葡萄的根、茎、叶、花蕾、盛花和胚珠中均有表达,但是有强弱差异。实时定量PCR技术分析表明在无核葡萄品种无核白胚珠发育过程中基因表达量先升后降,而在有核葡萄品种黑比诺胚珠发育过程中表达量一直呈下降趋势。
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| 关 键 词： | 葡萄 SVP类MADS-Box基因 表达分析 |
Cloning and Expression Analysis of Grape SVP-like MADS-box Gene |
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| YANG Kun
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ZHANG Chao-hong
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LI Shu-xiu
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ZHANG Xu-tong
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WANG Yue-jin.Cloning and Expression Analysis of Grape SVP-like MADS-box Gene[J].Journal of Northwest Forestry University,2012,27(4):117-123. |
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| Authors: | YANG Kun ZHANG Chao-hong LI Shu-xiu ZHANG Xu-tong WANG Yue-jin |
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| Institution: | (Colllege of Horticulture,Northwest A&F University,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northwest Region),Ministry of Agriculture,State Key Laboratory of Crop Stress Biology in Arid Areas,Yangling,Shaanxi 712100,China) |
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| Abstract: | A MADS-box gene associated with ovule development was cloned from Vitis.vinifera cv.Thompson seedless.The full-length of cDNA gene was 1 248 bp,including a 729 bp open reading frame,and encoding 243 amino acids.The homology and phylogenetic analysis showed that this gene belonged to SVP-like MADS-box genes.The gene was expressed in all of the grape tissue type examined by semi-quantity RT-PCR,including root,stem,leaf,bud,full bloom and ovules,but varied widely in the level of expression.Real-time PCR analysis revealed that gene expression level during ovule development in Thompson increased firstly and then decreased.In contrast to this,gene expression level in Pinot showed downward trend in all stages of development. |
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| Keywords: | Vitis SVP-like MADS-box gene expression analysis |
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