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木蹄层孔菌化学成分及不同提取物体外抗肿瘤活性研究
引用本文:陆勇芹,周文明,王琦,李玲玲.木蹄层孔菌化学成分及不同提取物体外抗肿瘤活性研究[J].西北林学院学报,2007,22(4):131-134.
作者姓名:陆勇芹  周文明  王琦  李玲玲
作者单位:1. 西北农林科技大学,生命科学学院,陕西,杨陵,712100
2. 西北农林科技大学,理学院,陕西,杨陵,712100
摘    要:采用石油醚、氯仿、乙酸乙酯、水饱和的正丁醇对木蹄层孔菌95%乙醇提取物进行分离,运用MTT比色法对各提取物进行体外抗肿瘤活性的测定。结果表明氯仿提取物对Hela细胞表现出较强的抑制作用,在测定浓度范围内呈现出良好的剂量依赖性抑制作用,而乙酸乙酯和正丁醇提取物在同一测定浓度范围内抑制作用不如氨仿提取物,其中石油醚提取物抑制作用最弱。化学预试表明,木蹄层孔菌中存在酚性成分,有机酸,糖、多糖和苷类,内酯、香豆素及其苷类,植物甾醇、萜类化合物,蒽醌及其苷类。

关 键 词:木蹄层孔菌  抗肿瘤活性  提取物  MTT法
文章编号:1001-7461(2007)04-0131-04
收稿时间:2007-01-22
修稿时间:2007-01-222007-03-28

A Preliminary Study on the Chemical Composition and Anticancer Effects of Fomes fomentarius in Vitro
LU Yong-qin,ZHOU Wen-ming,WANG Qi,LI Ling-ling.A Preliminary Study on the Chemical Composition and Anticancer Effects of Fomes fomentarius in Vitro[J].Journal of Northwest Forestry University,2007,22(4):131-134.
Authors:LU Yong-qin  ZHOU Wen-ming  WANG Qi  LI Ling-ling
Abstract:Different solvents were used to further fractionate 95% ethanol extract of Fomes fomentarius,i.e.,petro- leum ether,chloroform,ethyalacetate,and n-butanol.The anticancer activities of the different extracts in vitro were analyed with MTT method.The results showed that chloroform extract strongly inhibited proliferation of hela cells,and behaved significant does-dependent response while the ethyl acetate extract and the n-butanol extract ex- hibited low anticancer activities in vitro.The petroleum ether extract was the lowest one for its anticancer activity. Chemical compostions of F.formentarius were preliminarily examined.The results indicated that it contained phe- nols,organic acids,saccharides,polysaccharides,lactone,coumarinic lactone and its glycosides,steroids,triter- penoids,anthraquinones and its glycosides.
Keywords:Fomes fomentarius  anticancer activity  extract  MTT method
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