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| 污水、污泥中苏云金杆菌的分离及其基因型鉴定 | |
| 引用本文： | 吴丽云,何金清,周勇,陈国荣,张琳,张永嵘,吴松清,关雄.污水、污泥中苏云金杆菌的分离及其基因型鉴定[J].福建农林大学学报(自然科学版),2010,39(1). |
| 作者姓名： | 吴丽云 何金清 周勇 陈国荣 张琳 张永嵘 吴松清 关雄 |
| 作者单位： | 1. 福建农林大学生物农药与化学生物学教育部重点实验室,福建,福州,350002;湄洲湾职业技术学院,福建,莆田,351254 2. 福建莆田市秀屿区政府,福建,莆田,351146 3. 福建农林大学生物农药与化学生物学教育部重点实验室,福建,福州,350002 4. 英博雪津啤酒有限公司,福建,莆田,351111 |
| 基金项目： | 国家863计划(2006AA10A212);;教育部博士点基金(20060389011);;福建省科技创新平台建设计划(2007N2010);;莆田市科技计划(2008N18)资助项目 |
| 摘 要： | 从40个样品中分离到112株芽孢菌,其中有2株为Bt,占1.8%;从城市污水和啤酒厂生物处理系统中分别分离到1株Bt:采用cry1-cry11、cyt、vip3A、aiiA和inhA引物对BRC-WLY1和BRC-WLY2进行PCR扩增,发现2株Bt含有cry1(cry1Ag、cry1Ba、cry1Gb、cry1La)、cry2Ac、vip3A、aiiA杀虫基因,其中BRC-WLY1还含有inhA基因. |
| 关 键 词： | 苏云金芽孢杆菌 啤酒污水、污泥 分离 基因鉴定 |
Isolation and genotype identification of Bacillus thuringiensis from waste water and sludge |
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| WU Li-yun,HE Jin-qing,ZHOU Yong,CHEN Guo-rong,ZHANG Lin,ZHANG Yong-rong,WU Song-qing,GUAN Xiong.Isolation and genotype identification of Bacillus thuringiensis from waste water and sludge[J].Journal of Fujian Agricultural and Forestry University,2010,39(1). | |
| Authors: | WU Li-yun HE Jin-qing ZHOU Yong CHEN Guo-rong ZHANG Lin ZHANG Yong-rong WU Song-qing GUAN Xiong |
| Abstract: | One hundred and twelve colonies of Bacillus were isolated from 40 samples,of which 2 colonies(accounted for 1.8%) were B.thuringiensis. Among them one colony was Bt isolation,BRC-WLY1 was obtained from activated-sludge system environments from two urban sewage-digestive plants,and another Bt isolate(BRC-WLY2) was got from brewery sewage-digestive plant.The genes of cry1-cry11,cyt,vip3A,aiiA and inhA were amplified using specific primers.It showed that the two bacterial strains contained cry1(cry1Ag,cry1Ba,c... |
| Keywords: | Bacillus thuringiensis brewery sludge and water isolation gene identification |
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