| 应用蚕豆根尖微核技术研究3种重金属的遗传毒性 |
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| 引用本文： | 易志刚,傅巧姑.应用蚕豆根尖微核技术研究3种重金属的遗传毒性[J].福建农林大学学报(自然科学版),2009,38(4). |
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| 作者姓名： | 易志刚 傅巧姑 |
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| 作者单位： | 福建农林大学资源与环境学院,福建,福州,350002 |
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| 基金项目： | 福建农林大学青年教师科研基金资助项目(07A01) |
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| 摘 要： | 运用蚕豆根尖细胞微核技术,以微核率和有丝分裂指数为指标,研究不同浓度Pb2+、Cr3+和Cd2+对蚕豆根尖细胞的遗传毒性效应.结果表明,Pb2+、Cr3+和Cd2+在不同浓度下均可诱导微核的产生,其遗传毒性依次为:Cd2+>>Pb2+>Cr3+.在一定浓度范围内,微核率随重金属浓度的升高而增加,达到一定浓度后,微核率反而有下降的趋势.在一定低浓度条件下,蚕豆根尖细胞有丝分裂指数增高,高浓度下有丝分裂指数降低,意味着低浓度可促进细胞有丝分裂,高浓度则会抑制细胞有丝分裂.
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| 关 键 词： | 重金属 蚕豆 微核率 有丝分裂指数 |
Utilization of micronucleus test technique in Vicia faba root tips to evaluate the genetic toxicity of three heavy metal ions |
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| YI Zhi-gang,FU Qiao-gu.Utilization of micronucleus test technique in Vicia faba root tips to evaluate the genetic toxicity of three heavy metal ions[J].Journal of Fujian Agricultural and Forestry University,2009,38(4). |
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| Authors: | YI Zhi-gang FU Qiao-gu |
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| Institution: | College of Resources and Environment;Fujian Agriculture and Forestry University;Fuzhou;Fujian 350002;China |
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| Abstract: | Micronucleus test,micronucleus frequency and mitotic index of root tip cells of Vicia faba were used to study the genetic toxicity of different concentrations of heavy metal ions,namely Pb2+,Cr3+ and Cd2+.The results showed that different heavy metal ions could induce the occurrence of micronucleus.The highest genetic toxicity was found for Cd2+,the medium for Pb2+ and the lowest for Cr3+.Within certain concentrations of different heavy metals,micronucleus frequency increased with the increase of concentrat... |
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| Keywords: | heavy metal Vicia faba micronucleus frequency mitotic index |
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