褐牙鲆消化道粘液细胞的类型及分布

采用阿利新兰—过碘酸雪夫氏反应(AB-PAS)染色法和不同pH(1.0、2.5、3.1)的AB染色法,对褐牙鲆进行显微观察,发现褐牙鲆食管、胃、幽门垂和肠道均有粘液细胞分布.根据AB-PAS染色结果将褐牙鲆粘液细胞分成Ⅰ-Ⅳ4种类型:Ⅰ型呈红色,AB呈阴性反应,PAS呈阳性反应,含中性粘多糖;Ⅱ型呈蓝色,AB呈阳性反应,PAS呈阴性反应,含酸性粘多糖;Ⅲ型呈紫红色,AB和PAS均呈阳性反应,主要含有PAS呈阳性反应的中性粘多糖,同时含有少量AB呈阳性反应的酸性粘多糖;Ⅳ型呈蓝紫色,AB和PAS均呈阳性反应,主要含有AB呈阳性反应的酸性粘多糖,同时含有少量PAS呈阳性反应的中性粘多糖.AB(pH 2.5、3.1)染色显示,粘液细胞含酸性粘液(蓝色);AB(pH 1.0)染色显示,粘液细胞含弱的和强的硫酸化酸性粘液(蓝色).食管、胃、幽门垂和肠道含有大量粘液细胞,食管和中后肠以Ⅲ型和Ⅳ型细胞为主,幽门垂和前肠含有许多Ⅰ型细胞,胃只含大量的Ⅰ型细胞.通过对各部位粘液细胞的分类和比较可以看出,粘液细胞的分布和类型与其所在部位执行的功能有密切的关系.

Types and distribution of mucous cells in the digestive tract of Paralichthys olivaceus

Types and distribution of mucous cells in the digestive tract(oesophagus,stomach,pyloric caecum,intestine) of Paralichthys olivaceus were observed by the method of alcian blue and periodic acid Schiff′s reaction(AB-PAS) and alcine blue staining at different pH(1.0,2.5,3.1).According to AB-PAS,mucous cells were divided into four types.TypeⅠmucous cells were purely red,AB negative,PAS positive,containing neutral mucopolysaccharide.TypeⅡ mucous cells were purely blue,AB positive,PAS negative,containing acidic mucopolysaccharide.Type Ⅲ mucous cells were purple-red,both AB and PAS positive,mainly containing PAS positive neutral mucopolysaccharide and a little of AB positive acidic mucopolysaccharide.Type Ⅳ mucous cells were blue-purple,both AB and PAS positive,mainly containing acidic mucopolysaccharide and a little of AB positive neutral mucopolysaccharide.According to alcine blue staining at different pH,the mucous cells showed blue of the acidic mucopolysaccharide at pH 2.5 and pH 3.1,and showed weakly or powerful blue of vitriolic acidic mucopolysaccharide at pH 1.0.Based on the observation, there were abundant mucous cells in the oesophagus,stomach,pyloric caecum and intestine.Most of the mucous cells in the oesophagus,middle intestine and posterior intestine belonged to type Ⅲ and type Ⅳ cells,while most of the mucous cells in the pyloric caecum and anterior intestine belonged to typeⅠ cells.And there were only typeⅠcells in the stomach.Through the classification and the comparison of mucous cells,it could be concluded that there were close relationships between the distribution and types of the mucous cells and the functions at different locations.