| 黄花水仙和南日岛水仙的组培快繁 |
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| 引用本文： | 何玮毅,陈晓静,陈祥檀.黄花水仙和南日岛水仙的组培快繁[J].福建农林大学学报(自然科学版),2005,34(3):313-317. |
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| 作者姓名： | 何玮毅 陈晓静 陈祥檀 |
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| 作者单位： | 福建农林大学园艺学院,福建,福州,350002 |
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| 摘 要： | 以黄花水仙和南日岛水仙为材料,对2个水仙品种的组培快繁技术进行了研究.结果表明:以低温处理1个月的带鳞茎盘基部的鳞片为外植体,接种于MS+0.5 mg.L-1BA+0.1 mg.L-12,4-D的诱导培养基和MS+1.0 mg.L-1BA+0.5或1.0 mg.L-12,4-D的继代增殖培养基中,诱导黄花水仙形成的小鳞茎数量多,增殖率高;南日岛水仙以MS+0.5 mg.L-1BA+2.0 mg.L-1NAA的诱导培养基和MS+1.0 mg.L-1BA+0.5或1.0 mg.L-12,4-D的增殖培养基效果较好.生根培养基为1/2MS+0.05 mg.L-1NAA,移栽后成活率很高.
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| 关 键 词： | 中国水仙 组织培养 快速繁殖 |
| 文章编号： | 1671-5470(2005)03-0313-05 |
| 收稿时间： | 2005-04-19 |
| 修稿时间： | 2005-06-30 |
In vitro propagation of Huanghua and Nanridao narcissus |
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| HE Wei-yi,CHEN Xiao-jing,CHEN Xiang-tan.In vitro propagation of Huanghua and Nanridao narcissus[J].Journal of Fujian Agricultural and Forestry University,2005,34(3):313-317. |
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| Authors: | HE Wei-yi CHEN Xiao-jing CHEN Xiang-tan |
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| Abstract: | The study was carried out to develop a technological system for rapid propagation of Huanghua and Nanridao narcissus in vitro.The result indicated that segments of the bulbs with basal scales and a portion of the plate,which were pretreated under 4 ℃ for a month,were well used as explants.MS medium containing 0.5 mg·L~(-1) BA and 0.1 mg·L~(-1) 2,4-D or 0.5 mg·L~(-1) BA and 2.0 mg·L~(-1) NAA was suitable for induction of bulblets of Huanghua or Nanridao narcissus,respectively.The MS medium,supplemented with 1.0 mg·L~(-1) BA and 0.5 mg·L~(-1) or 1.0 mg·L~(-1) 2,4-D,could be used for subculture of Huanghua and Nanridao narcissus.A high rate of viability was obtained in a rooting induction medium of 1/2 MS added with 0.05 mg·L~(-1) NAA for both narcissus. |
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| Keywords: | Narcissus tazetta Linn var chinensis Roem in vitro culture rapid propagation |
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