| 苏云金芽孢杆菌NAD(P)H:醌氧化还原酶基因的克隆和敲除载体的构建 |
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| 引用本文： | 李今煜,肖水华,黄天培.苏云金芽孢杆菌NAD(P)H:醌氧化还原酶基因的克隆和敲除载体的构建[J].福建农林大学学报(自然科学版),2012,41(5):508-512. |
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| 作者姓名： | 李今煜 肖水华 黄天培 |
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| 作者单位： | 福建农林大学生物农药与化学生物学教育部重点实验室,福建福州,350002 |
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| 基金项目： | 国家自然科学基金，国家863项目，福建省高校服务海西建设重点项目 |
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| 摘 要： | 以苏云金芽孢杆菌库斯塔克亚种(Bacillus thuringiensis subsp.kurstaki)8010为研究材料,通过PCR技术获得Bt 8010菌株NAD(P)H:醌氧化还原酶基因上下游2个片段,以含有卡那霉素抗性基因的pDG780质粒为中间载体,通过酶切、连接和PCR,构建含待敲除NAD(P)H:醌氧化还原酶基因上下游片段、卡那霉素抗性基因的重组质粒pRN5101DKU,为后续Bt8010菌株NAD(P)H:醌氧化还原酶基因的敲除做准备.
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| 关 键 词： | 苏云金芽孢杆菌 NAD(P)H:醌氧化还原酶基因 基因敲除载体 |
Cloning and knockout vector construction of a NAD(P)H: quinone oxidoreductase gene in Bacillus thuringiensis |
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| LI Jin-yu
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XIAO Shui-hua
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HUANG Tian-pei.Cloning and knockout vector construction of a NAD(P)H: quinone oxidoreductase gene in Bacillus thuringiensis[J].Journal of Fujian Agricultural and Forestry University,2012,41(5):508-512. |
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| Authors: | LI Jin-yu XIAO Shui-hua HUANG Tian-pei |
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| Institution: | (Key Laboratory of Biopesticide and Chemical Biology,Ministry of Education, Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China) |
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| Abstract: | Bacillus thuringiensis subsp.kurstaki isolate 8010 was selected to obtain the upstream and downstream sequences of NAD(P)H: quinone oxidoreductase gene by PCR technology.The pDG780 vector(containing the kanamycin resistance gene) was used to construct a recombination plasmid pRN5101DKU which contained the upstream and downstream sequences of NAD(P)H: quinone oxidoreductase,and the kanamycin resistance gene by PCR and RE treatments.It was provided for further study on gene knockout. |
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| Keywords: | Bacillus thuringiensis NAD(P)H: quinone oxidoreductase gene gene knockout vector |
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