红车轴草提取物对脂多糖应激断奶仔猪生长性能和炎性介质的影响

为研究红车轴草提取物(RCE)对脂多糖(LPS)应激的断奶仔猪生长性能和炎性介质的影响,本试验选用30头健康、平均体重为(6.86±0.14)kg的(21±1)日龄(杜×长×大)断奶仔猪,按照随机区组设计设5个处理,每个处理6个重复,每个重复1头仔猪.5个处理组仔猪分别饲喂基础日粮(非应激空白对照组)、基础日粮+LPS(LPS组)、基础日粮+LPS+0.05%RCE(0.05%RCE组)、基础日粮+LPS+0.10%RCE(0.10%RCE组)、基础日粮+LPS+0.20%RCE(0.20%RCE组).试验期21 d.在试验第7、14天,LPS组和0.05%、0.10%、0.20%RCE组仔猪每千克体重注射80μg LPS,空白对照组注射等量的生理盐水.结果表明:(1)试验第1-7天(即LPS应激前),与空白对照组、LPS组相比,0.10%RCE组仔猪的日增重显著升高(P<0.05),料重比显著降低(P<0.05),而0.05%和0.20%RCE组的日增重和料重比与空白对照组、LPS组相比无显著差异(P>0.05);试验第7-21天(即注射LPS两次之后),与空白对照组相比,LPS组的日增重和日采食量显著降低(P<0.05),料重比显著提高(P<0.05),而0.05%、0.10%和0.20%RCE组的日增重和日采食量有所下降,但差异不显著(P>0.05).(2)在第7、14天注射LPS后,与空白对照组相比,LPS组仔猪血清中的IgG含量显著降低(P<0.05),血清中白介素-6(IL-6)和肿瘤坏死因子TNF-α的含量均显著提高(P<0.05).与LPS组相比,0.05%RCE组的IgG、IL-6和TNF-α含量差异不显著(P>0.05);0.10%和0.20%RCE组的IgG含量均显著提高P<0.05),IL-6和TNF-α含量均显著降低(P<0.05);以添加0.10%RCE缓解仔猪应激效果最为明显,与LPS组相比,IgG含量提高38.65%(P<0.05),IL-6含量降低32.81%(P<0.05),TNF-α含量降低24.68%(P<0.05).可见,日粮中添加RCE可在一定程度上缓解LPS刺激导致的断奶仔猪生长性能和炎性细胞因子分泌的改变,以添加0.10%RCE效果最好.

Effect of red clover extract on growth performance and inflammatory parameters of weaned pigs with lipopolysaccharide challenging

To study the effects of red clover extract(REC) on growth performance and inflammatory parameters of weaned pigs with lipopolysaccharide(LPS) challenging.Thirty healthy Duroc×Landrace×Yorkshire crossbred weaned piglets with body weight of(6.86±0.14) kg were randomly allocated to 5 groups.Every group included 6 replicates and 1 piglet in per replicate.The five treatments groups include: basic ration groups(no LPS control groups),basic ration groups+LPS control groups(LPS control groups),basic ration groups+LPS control groups+0.05% RCE groups(0.05% RCE groups),basic ration groups+LPS control groups+0.10% RCE groups(0.10% RCE groups),basic ration groups+LPS control groups+0.20% RCE groups(0.20% RCE groups).On the 21st day after weanling,experiment piglets in groups LPS,0.05% RCE,0.10% RCE and 0.20% RCE were injected intraperitoneally with 80 μg·kg-1 LPS every other day(on day 7,7 and 14 of experiment),whereas pigs in groups 1 were injected with an equivalent amount of sterile saline.The results showed:(1) Compared with the blank control groups and LPS groups,from day 1 to 7(before LPS challenge),the average daily gain of piglets in the 0.10% RCE groups increased significantly(P<0.05) and average feed-gain ratio decreased significantly(P<0.05).While there was no significant difference among 0.05% RCE groups,0.20% RCE groups and control groups on average daily gain of piglets and feed/gain ratio(P>0.05).From day 7 to 21(with LPS challenge twice),the average daily gain of piglets and average daily feed intake in LPS groups decreased significantly(P<0.05) and average feed-gain ratio increased significantly(P<0.05) compared with the blank control groups.While the average daily gain of piglets and average daily feed intake in 0.05% REC groups,0.1% REC groups,0.20% RCE groups decreased,but not significantly(P>0.05).(2) After injected intraperitoneally with LPS every other day(on day 7 and 14 of experiment),the serumal IgG levels of piglets in the LPS groups were significantly lower than those in control groups(P<0.05),while the serumal cytokine IL-6 and tumor necrosis factor TNF-α were both increased significantly(P<0.05).There was no significant difference between 0.05% RCE groups and LPS groups on serumal IgG levels,serumal cytokine IL-6 and tumor necrosis factor TNF-α(P>0.05).The serumal IgG levels of piglets in the 0.1% REC groups and 0.2% REC groups increased significantly(P<0.05),but the serumal cytokine IL-6 and tumor necrosis factor TNF-α both decreased significantly(P>0.05).The dietary supplementation with 0.1% REC could significantly relieve the weaned piglets challenged by LPS.Compared with the LPS groups,the serumal IgG levels increased by 38.65%(P<0.05),serumal cytokine IL-6 decreased by 32.81%(P<0.05) and TNF-α both decreased by 24.68%(P<0.05).The result indicated that the dietary supplementation with 0.1% REC was best,it can alleviate the growth depression and inflammatory cytokine secretion change of weaned piglets challenged by LPS to some degree.