| 水稻瘤矮病毒S3和S8基因共表达杆状病毒转移载体构建及重组病毒的鉴定 |
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| 引用本文： | 范国成,高芳銮,黄美英,谢荔岩,吴祖建,林奇英,谢联辉.水稻瘤矮病毒S3和S8基因共表达杆状病毒转移载体构建及重组病毒的鉴定[J].福建农林大学学报(自然科学版),2011(2):151-155. |
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| 作者姓名： | 范国成 高芳銮 黄美英 谢荔岩 吴祖建 林奇英 谢联辉 |
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| 作者单位： | 福建农林大学植物病毒研究所;福建省农业科学院果树研究所;福建省植物病毒学重点实验室; |
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| 基金项目： | 福建省自然科学基金计划资助项目(2006J0065、2009J01046); 青年教师科研基金计划项目(2010004) |
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| 摘 要： | 为构建携带水稻瘤矮病毒(Rice gall dwarf virus,RGDV)主要内层衣壳蛋白S3基因和外层衣壳蛋白S8基因的重组杆状病毒,将目的基因(S3和S8)分别亚克隆到杆状病毒转移载体pFastBacDual多角体启动子(PH)和p10启动子的下游.经酶切和确证性序列测定,将其转化到DH10Bac感受态细胞中,...
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| 关 键 词： | 水稻瘤矮病毒 外层衣壳蛋白 内层衣壳蛋白 杆状病毒表达系统 Sf9昆虫细胞 |
Construction of baculovirus transfer vector for co-expression of Rice gall dwarf virus gene S3 and S8 and identification of recombinant baculovirus |
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| FAN Guo-cheng,GAO Fang-luan,HUANG Mei-ying,XIE Li-yan,WU Zu-jian,LIN Qi-ying,XIE Lian-hui.Construction of baculovirus transfer vector for co-expression of Rice gall dwarf virus gene S3 and S8 and identification of recombinant baculovirus[J].Journal of Fujian Agricultural and Forestry University,2011(2):151-155. |
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| Authors: | FAN Guo-cheng GAO Fang-luan HUANG Mei-ying XIE Li-yan WU Zu-jian LIN Qi-ying XIE Lian-hui |
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| Institution: | FAN Guo-cheng1,2,GAO Fang-luan1,3,HUANG Mei-ying1,XIE Li-yan1,WU Zu-jian1,LIN Qi-ying1,XIE Lian-hui1,3 (1.Institute of Plant Virology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China,2.Research Institute of Fruits,Fujian Academy of Agricultural Sciences,Fujian 350013,3.Key Lab for Plant Virology of Fujian Province,China) |
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| Abstract: | To construct a recombinant baculovirus co-expressing the major core capsid protein gene S3 and outer capsid protein gene S8 of Rice gall dwarf virus(RGDV),the target genes(S3 and S8) were subcloned into the downstream of PH promoter and p10 promoter of the baculovirus transfer vector pFastBacDual,respectively.After transformation,pFBDS3-S8,which was identified with restriction enzyme digestion and conformed by sequence analysis,was introduced into the competent cells(Escherichia coli DH10Bac),generating the... |
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| Keywords: | Rice gall dwarf virus(RGDV) outer capsid protein core capsid protein baculovirus expression system Spodoptera frugiperda(Sf9) insect cell |
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