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乌龙茶品种武夷名丛成年材料的离体培养Ⅰ.成年树茎段无菌系的建立
引用本文:陈菁,赖钟雄,孙威江,郭玉琼.乌龙茶品种武夷名丛成年材料的离体培养Ⅰ.成年树茎段无菌系的建立[J].福建农林大学学报(自然科学版),2011(3):257-263.
作者姓名:陈菁  赖钟雄  孙威江  郭玉琼
作者单位:福建农林大学园艺学院;
基金项目:中国科学技术部资助项目(2010GA720012); 省长专项基金资助项目(闽财指[2008]511号); 福建省科技厅资助项目(2008S0101)
摘    要:以乌龙茶品种武夷名丛中的早生种金凤凰、中生种金锁匙和晚生种肉桂的成年树茎段为外植体,比较外植体灭菌、切割方式和外源植物激素、盐、蔗糖以及光照度等因素对腋芽诱导的影响.结果表明:春季一芽二叶时期二叶位的茎段做为外植体较为合适;用0.1%HgCl浸泡9-11min为最佳消毒方式,但应视茶树的品种(系)以及茎段的老嫩程度而定...

关 键 词:茶树  武夷名丛  离体培养  成年茎段  无菌系

In vitro culture of adult stems of Wuyimingcong cultivar group Ⅰ.Establishment of asepsis system of mature stem section
CHEN Jing,LAI Zhong-xiong,SUN Wei-jiang,GUO Yu-qiong.In vitro culture of adult stems of Wuyimingcong cultivar group Ⅰ.Establishment of asepsis system of mature stem section[J].Journal of Fujian Agricultural and Forestry University,2011(3):257-263.
Authors:CHEN Jing  LAI Zhong-xiong  SUN Wei-jiang  GUO Yu-qiong
Institution:CHEN Jing,LAI Zhong-xiong,SUN Wei-jiang,GUO Yu-qiong(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)
Abstract:In this experiment the adult stem nodes were used as materials to carry out in vitro culture of Wuyimingcong cultivar group such as the early-sprouting line Jinfenghuang,the middle-sprouting line Jinsuoshi and the late-sprouting cultivar Rougui.The induction effects of sterilization methods,cutting mode,light intensity,types of growth hormone,salt and sugar concentration on Wuyimingcong axillary buds were investigated.The results showed that the most suitable explants were two-leaf-located stem materials in...
Keywords:Camellis sinenesis  Wuyimingcong cultivar group  In vitro culture  matural stem meristem  asepsis system  
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