| 甘露糖对甘蔗外植体再生的影响 |
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| 引用本文： | 吴杨,傅华英,赵书环,陈如凯,张木清. 甘露糖对甘蔗外植体再生的影响[J]. 福建农林大学学报(自然科学版), 2008, 37(3): 225-229 |
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| 作者姓名： | 吴杨 傅华英 赵书环 陈如凯 张木清 |
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| 作者单位： | 1. 福建农林大学农业部甘蔗生理生态与遗传改良重点实验室,福建,福州,350002;井冈山大学生命科学学院,江西,吉安,343009
2. 福建农林大学农业部甘蔗生理生态与遗传改良重点实验室,福建,福州,350002 |
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| 基金项目： | 国家高技术研究发展计划(863计划) , 福建省自然科学基金 |
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| 摘 要： | 在甘蔗外植体再生体系的基础上,在甘蔗愈伤组织继代、胚性愈伤组织芽分化以及幼芽生根阶段的培养基中附加甘露糖,研究甘露糖对甘蔗外植体再生的影响,以确定用于甘蔗遗传转化筛选的最适浓度.结果表明,甘露糖对甘蔗茎尖愈伤组织诱导、愈伤分化和幼芽根形成均产生抑制作用.愈伤组织生长、分化、生根3个阶段的抗性筛选浓度分别为3-5、4-5和3 g.L-1.
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| 关 键 词： | 甘蔗愈伤 甘露糖 筛选体系 |
| 文章编号： | 1671-5470(2008)03-0225-05 |
| 修稿时间： | 2007-05-09 |
Effects of mannose on in vitro plant regeneration of Saccharum officinarum |
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| WU Yang,FU Hua-ying,ZHAO Shu-huan,CHEN Ru-kai,ZHANG Mu-qing. Effects of mannose on in vitro plant regeneration of Saccharum officinarum[J]. Journal of Fujian Agricultural and Forestry University, 2008, 37(3): 225-229 |
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| Authors: | WU Yang FU Hua-ying ZHAO Shu-huan CHEN Ru-kai ZHANG Mu-qing |
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| Abstract: | The study was based on the regeneration system of sugarcane established by Key Lab of Eco-physiology & Genetic Improvement for Sugarcane,Ministry of Agriculture.Mannose of different concentrations was added into each medium of sugarcane tissue culture during secondary,regeneration and rootage stages.Moreover,the effect of mannose on explants regeneration was analyzed,so that the optimum screening mass-concentrations needed by genetic transformation of sugarcane could be determined.The results indicated that mannose had inhibiting effect on induction frequencies of calli,shoots and rooting.The screening concentrations of mannose at the subculture,regeneration and rootage stages of sugarcane callus were 3-5,4-5 and 3 g·L-1,respectively. |
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| Keywords: | sugarcane callus mannose screening system |
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