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蜡蚧轮枝菌高产毒素菌株的筛选
引用本文:王联德,黄建.蜡蚧轮枝菌高产毒素菌株的筛选[J].福建农林大学学报(自然科学版),2006,35(2):134-137.
作者姓名:王联德  黄建
作者单位:福建农林大学生物农药与化学生物学教育部重点实验室,福建,福州,350002
基金项目:中国科学院资助项目;教育部重点实验室基金;福建省青年科技人才创新基金
摘    要:通过比较6株蜡蚧轮枝菌(V3450、Vp28、V l6063、V0175、V342和V341)对烟粉虱3龄若虫的毒力和6株菌株代谢浓缩液对烟粉虱1龄若虫的忌避作用和毒杀作用,筛选出高产毒素菌株.结果表明:6株蜡蚧轮枝菌的LC50分别为2.57×105、6.03×105、6.03×105、1.70×106、7.41×106、1.66×107孢子.mL-1,表明菌株V3450、Vp28、V l6063的毒力比另外3株高;6株菌株代谢浓缩液对烟粉虱1龄若虫的忌避率分别为76.40%、78.22%、73.54%、52.7%、40.80%、53.33%,死亡率分别为80.27%、71.90%、66.66%、50.86%、40.57%、46.67%,表明菌株V3450、Vp28、V l6063的产毒能力比另外3株强,由此筛选出V3450、Vp28、V l6063为高产毒素菌株.

关 键 词:蜡蚧轮枝菌  毒素  毒力  筛选
文章编号:1671-5470(2006)02-0134-04

Selection of Verticillium lecanii strains for high production of toxin
WANG Lian-de,HUANG Jian.Selection of Verticillium lecanii strains for high production of toxin[J].Journal of Fujian Agricultural and Forestry University,2006,35(2):134-137.
Authors:WANG Lian-de  HUANG Jian
Abstract:Verticillium lecanii strains with higher toxin production abilities were selected based on the comparisons of the virulence of 6 strains of V.lecanii(V3450,Vp28,Vl6063,V0175,V342,and V341)and effects of dilutions of the crude extract from these 6 strains on nymphs of B.tabaci being conducted by the bioassay approach.The results were as follows: the strain Vl6063 from Canada and the domestic strains V3450,Vp28 derived from B.tabaci and scale insect,respectively,were more virulent than the others with the LC_(50) values of 2.57×10~5,6.03×10~5 and 6.03×10~5 conidia·mL~(-1),respectively;the dilutions of crude extracts from the strains V3450,Vp28 and Vl6063 were more virulent than the other 3 strains.The mortalities to the 1~(st) instar of B.tabaci were(80.27%),71.90% and 66.66%,respectively.
Keywords:Verticillium lecanii  toxin  virulence  selection
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