福建东方蜜蜂线粒体DNA的遗传变异和遗传多样性

为了研究福建东方蜜蜂的遗传变异和遗传多样性,在全省采集9个样点,共424群东方蜜蜂样本,进行线粒体DNA tRNAleu-COⅡ序列分析.结果表明,tRNAleu-COⅡ片段序列包括93 bp的非编码区和259 bp的COⅡ编码区.在整个352 bp序列中,共检测到23个多态位点和27种单倍型,平均单倍型多样度为0.484 3,平均核苷酸差异数为0.687,平均核苷酸多样度为0.001 93.福建东方蜜蜂各样点间不存在遗传分化,总体遗传分化系数为0.003,各样点的基因流参数为49.75~249.75.

Genetic variation and genetic diversity of Apis cerana from Fujian province based on mitochondrial DNA sequence analysis

To examine the genetic variation and diversity of Apis cerana in Fujian province, sequence analysis of mitochondrial DNA fragments of tRNAleu-COⅡin honeybees was conducted. These honeybees were sampled from 424 colonies from 9 cities cross Fujian province. The sequence was 352 bp at length, consisting of a 93 bp non-coding region and a 259 bp partial COⅡ coding region. A total of 23 polymorphic sites and 27 haplotypes were found in 424 sequences. Haplotype diversity and nucleotide difference averaged at 0.484 3 and 0.687, with nucleotide diversity of the samples being 0.001 93. The overall genetic differentiation coefficient was 0.003, with gene flow ranging from 49. 75 to 249. 75. To summarize, no population differentiation was detected among honeybees across Fujian province.