| 苏云金芽孢杆菌外孢囊蛋白基因敲除载体的构建 |
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| 引用本文： | 李今煜,潘智雄,黄天培.苏云金芽孢杆菌外孢囊蛋白基因敲除载体的构建[J].福建农林大学学报(自然科学版),2012,41(2):159-162. |
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| 作者姓名： | 李今煜 潘智雄 黄天培 |
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| 作者单位： | 福建农林大学生物农药与化学生物学教育部重点实验室,福建福州,350002 |
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| 基金项目： | 福建省高校服务海西建设重点项目，福建省教育厅科技项目 |
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| 摘 要： | 以苏云金芽孢杆菌(Bt)库斯塔克亚种(subsp.kurstaki) 8010为研究材料,通过PCR技术获得Bt8010的外孢囊蛋白基因上下游序列,以含有卡那基因的pDG780质粒为中间载体,通过酶切、连接和PCR,构建含外孢囊蛋白基因上游序列、卡那基因、外孢囊蛋白基因下游序列的基因敲除载体pRN5101UKD,为后续...
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| 关 键 词： | 苏云金芽孢杆菌 外孢囊蛋白基因 基因敲除载体 |
Construction of the exosporium protein gene knockout vector in Bacillus thuringiensis |
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| LI Jin-yu
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PAN Zhi-xiong
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HUANG Tian-pei.Construction of the exosporium protein gene knockout vector in Bacillus thuringiensis[J].Journal of Fujian Agricultural and Forestry University,2012,41(2):159-162. |
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| Authors: | LI Jin-yu PAN Zhi-xiong HUANG Tian-pei |
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| Institution: | (Key Laboratory of Biopesticide and Chemical Biology,Ministry of Education,Fujian Agriculture and Forestry University, Fuzhou,Fujian 350002,China) |
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| Abstract: | Bacillus thuringiensis subsp.kurstaki isolate 8010 was selected to obtain the upstream and downstream of exosporium protein gene by PCR technology.The pDG780 vector(containing the kanamycin resistance gene) was used to construct a recombination plasmid pRN5101UKD which contained the upstream sequence of exosporium protein gene,the kanamycin resistance gene and the downstream sequence of exosporium protein gene by PCR and RE treatments.It was provided for further study on gene knockout. |
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| Keywords: | Bacillus thuringiensis exosporium protein gene gene knockout vector |
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