淡紫拟青霉FZ-0289产几丁质酶的条件优化

通过单因素和多因素正交试验对淡紫拟青霉FZ0289产几丁质酶的条件进行了研究.单因素试验表明:100目几丁质粉末对菌体产几丁质酶的诱导性最强;添加蛋白胨能使产酶高峰提前,产酶量增加.多因素正交试验表明,在添加2g·L-1100目几丁质粉末、2g·L-1蛋白胨、1g·L-1KH2PO4、0.5g·L-1MgSO4、0.2g·L-1CaCl2和0.2g·L-1NaCl的培养基中接入经马铃薯蔗糖培养基(PSA)活化10d的菌碟(接菌孢子量为6×109个·L-1,500mL摇瓶中培养体积为150mL),在28℃、150r·min-1的条件下培养3d,发酵液中几丁质酶活达到0.11U·mL-1.

Optimizing conditions of chitinase production from Paecilomyces lilacinus strain FZ-0289

Single-factor design and multiple factor design were used for optimiz ing cultural conditions of chitinase production from Paecilomyces lilacinus stra in FZ-0289. Single-factor design showed that chitin powder (165 μm) was the b est medium for inducing the chitinase from FZ-0289, and by adding peptone to c hitin powder the peak of chitinase production could appeared ahead of time. Mult iple factor design showed that optimal cultural medium for chitinase production included 2 g·L~-1 chitin powder (165 μm), 2 g·L~-1 peptone, 1 g·L~-1 KH2PO4, 0 .5 g·L~-1 MgSO4, 0.2 g·L~-1 CaCl2 and 0.2 g·L~-1 NaCl. The FZ-0 289 strain was activated on potato sugar agar (PSA) plates for 10 days, then was inoculat ed with 6×10~9 spores·L~- 1 in 150 mL optimal liquid medium. After fermented at 28 ℃ with the revolving speed of 150 r·min~-1 in 500 mL conical-flask, the chitinase activity co uld reach 0.11 U·mL~-1 on the third day of incubation period.