| 生物工程应用于作物育种的试验研究 第2报 外源DNA处理蚕豆的细胞学和同工酶的若干效应 |
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| 引用本文： | 朱秀英,陈璋,卢勤,林学健.生物工程应用于作物育种的试验研究 第2报 外源DNA处理蚕豆的细胞学和同工酶的若干效应[J].福建农林大学学报(自然科学版),1988(1). |
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| 作者姓名： | 朱秀英 陈璋 卢勤 林学健 |
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| 作者单位： | 福建农学院作物遗传育种研究所，福建农学院作物遗传育种研究所，福建农学院作物遗传育种研究所，福建农学院作物遗传育种研究所 |
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| 摘 要： | 以外源DNA处理蚕豆萌动种子,观察根尖,芽尖分裂细胞的染色体畸变,分析根芽的过氧化物酶同工酶(POD)和脂酯同工酶(EST).结果表明。DNA 处理能引起落后断片、染色体桥等染色体畸变,畸变率的多少与 DNA 的浓度无关。DNA 处理也使过氧化物酶同工酶带数及酶活性有增加的趋势,脂酶同工酶的活性却受到抑制。
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| 关 键 词： | 外源DNA 蚕豆 根 芽 染色体畸变 过氧化物酶同工酶 脂酶同工酶 酶活性 |
STUDY ON THE BIOTECHNOLOGY APPLIED IN CROP BREEDING Ⅱ.The Effect of Exogenous DNA on the Cytology and Isozyme in Vicia faba |
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| Zhu Xiuying Chen Zhang Lu Qin Lin Xuejian.STUDY ON THE BIOTECHNOLOGY APPLIED IN CROP BREEDING Ⅱ.The Effect of Exogenous DNA on the Cytology and Isozyme in Vicia faba[J].Journal of Fujian Agricultural and Forestry University,1988(1). |
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| Authors: | Zhu Xiuying Chen Zhang Lu Qin Lin Xuejian |
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| Abstract: | The cytological effect and isozyme alterations were studied by applying exogenous DNA cattle pancreas(a Shanghai Milk Co.product),to the sprouted seeds of Vicice faba.The results were shown as follows:(1) Application of exogenous DNA in the germinating Vicia faba affected the chromosomal aberration,including chromosome fragment,chromosome bridge etc There were found in the cells of root and bud.The aberration ratio was not interrelated to the concentration of DNA(2)The number of isoperoxidase bands and isoperoxidase activities of the treated bud and root increased in comparison with the CK.On the contrary,the activity of esterase isozyme was restrained. |
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| Keywords: | exogenous DNA Vicia faba root bud chromosomal aberration isoperoxidase esterase isozyme enzyme activity |
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