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| 稻瘟菌若干T-DNA插入突变体初步分析 | |
| 引用本文： | 魏艺聪,潘初沂,李宏宇,鲁国东,雷财林,王宗华.稻瘟菌若干T-DNA插入突变体初步分析[J].福建农林大学学报(自然科学版),2007,36(1):47-50. |
| 作者姓名： | 魏艺聪 潘初沂 李宏宇 鲁国东 雷财林 王宗华 |
| 作者单位： | 1. 福建农林大学功能基因组学研究中心,福建,福州,350002 2. 福建农林大学功能基因组学研究中心,福建,福州,350002;中国农业科学院重大工程中心,北京,100081 3. 中国农业科学院重大工程中心,北京,100081 |
| 基金项目： | 国家自然科学基金 , 福建省自然科学基金 |
| 摘 要： | 为揭示稻瘟菌致病分子机制,创建了该菌T-DNA插入突变体库．部分突变体经表型分析,获得3个生长发育及致病性同时发生变异,5个生长发育正常但致病性变异及2个生长发育变异但致病性正常的突变体；以TAIL-PCR方法获得了这些突变体T-DNA插入位点及其边界序列,并用生物信息方法对被T-DNA标签的基因进行了分析,为进一步研究这些基因的功能提供了重要信息． |
| 关 键 词： | 稻瘟菌 T-DNA插入变异 生物信息 |
| 文章编号： | 1671-5470(2007)01-0047-04 |
| 修稿时间： | 2006-10-12 |
Analysis of Magnaporthe grisea Mutants through T-DNA Insertion |
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| WEI Yi-Cong,PAN Chu-yi,LI Hong-yu,LU Guo-dong,LEI Cai-lin,WANG Zong-hua.Analysis of Magnaporthe grisea Mutants through T-DNA Insertion[J].Journal of Fujian Agricultural and Forestry University,2007,36(1):47-50. | |
| Authors: | WEI Yi-Cong PAN Chu-yi LI Hong-yu LU Guo-dong LEI Cai-lin WANG Zong-hua |
| Institution: | 1. The Functional Genomics Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 2. The National Key Engineering Center, Chinese Academy of Agricultural Sciences, Beijing 100080, China |
| Abstract: | To explore molecular mechanism in regulating pathogenesis of the rice blast fungus, Magnaporthe grisea, a T-DNA inser-tional mutant library of the fungus was constructed. Partial mutants in the library were selected to conduct phenotype and genetics characterization. Among these, 3 mutants showed mutation both in growth and pathogenicity, 5 showed normal in growth but mutated in pathogenecity, while other 2 showed mutated in growth but normal in pathogenecity. The T-DNA flanking region of these mutants was further amplified by TAIL-PCR. The T-DNA target genes were applied to analyze by bioinformatics methods. These data will provide us important information to further study the function of these genes. |
| Keywords: | Magnaporthe grisea T-DNA insertional mutagenesis j bioinformatics |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |