荔枝胚状体POD与EST同工酶酶谱分析 |
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引用本文: | 黄素华,赖钟雄.荔枝胚状体POD与EST同工酶酶谱分析[J].福建农林大学学报(自然科学版),2002,31(4):467-469. |
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作者姓名: | 黄素华 赖钟雄 |
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作者单位: | 福建农林大学亚热带果树研究所,福建,福州,350002 |
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基金项目: | 国家自然科学基金(39870544),霍英东高校青年教师基金(71026)资助项目的部分内容. |
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摘 要: | 采用不连续垂直平板聚丙烯酰胺凝胶电泳,对荔枝"元红"品种幼胚胚性愈伤组织诱导出的胚状体进行过氧化物酶同工酶和酯酶同工酶谱分析.结果表明:染色方法对同工酶谱谱带有影响;同一发育阶段的荔枝胚状体的过氧化物酶与酯酶同工酶谱有一定差异,表明不同胚状体之间可能已产生一定的遗传变异.
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关 键 词: | 胚状体 过氧化物酶同工酶 酯酶同工酶 变异 |
文章编号: | 1006-7817(2002)04-0467-03 |
修稿时间: | 2001年11月30 |
Analysis of isoenzymatic patterns of POD and EST of somatic embryos in litchi |
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Abstract: | Isoenzymatic patterns of POD and EST of somatic embryos in Litchi chinenesis cv. Yanhong were analysed through discontinuous polyacrylamide gel electrophoresis. The results showed that the staining methods affected the strength of the belt activity and the number of the belts of the isoenzymatic patterns, and some differences in the isoenzymatic patterns of POD and EST occurred among somatic embryos at the same developmental stage, which suggested that some genetic variations existed among the different somatic embryos. |
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Keywords: | somatic embryo POD isoenzyme EST isoenzyme variation |
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