| 产碱性蛋白酶海洋细菌的筛选与鉴定 |
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| 引用本文： | 黄志强,林白雪,谢联辉.产碱性蛋白酶海洋细菌的筛选与鉴定[J].福建农林大学学报(自然科学版),2006,35(4):416-420. |
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| 作者姓名： | 黄志强 林白雪 谢联辉 |
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| 作者单位： | 1. 福建农林大学生物农药与化学生物学教育部重点实验室,福建,福州,350002
2. 福建农林大学植物病毒研究所,福建,福州,350002 |
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| 基金项目： | 福建省自然科学基金;福建省科技攻关项目 |
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| 摘 要： | 从福建海域的海水、海泥及海藻样品中分离到3株产碱性蛋白酶的海洋细菌菌株3B、6CW、15E,采用16S rDNA分子生物学鉴定结合细菌的常规鉴定方法,确认分离到的3株产蛋白酶的海洋细菌菌株分别是荧光假单胞菌(Pseudom onas fluo-rescens)、粘质沙雷菌(Serratia marcescens)和氧化短杆菌(Brevibacterium oxydans),并对其发酵液中的蛋白酶活力做了初步研究.
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| 关 键 词： | 海洋细菌 蛋白酶 筛选 鉴定 |
| 文章编号： | 1671-5470(2006)04-0416-05 |
| 收稿时间： | 2005-11-13 |
| 修稿时间： | 2006-06-19 |
Isolation and identification of alkaline protease-producing marine bacteria |
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| HUANG Zhi-qiang,LIN Bai-xue,XIE Lian-hui.Isolation and identification of alkaline protease-producing marine bacteria[J].Journal of Fujian Agricultural and Forestry University,2006,35(4):416-420. |
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| Authors: | HUANG Zhi-qiang LIN Bai-xue XIE Lian-hui |
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| Institution: | Key Laboratory of Biopesticide and Chemical Biology of Ministry of Education ; Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China |
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| Abstract: | Three alkaline protease-producing marine bacterial strains,named as 3B,6CW and 15E,were isolated from samples of sea-water,sea-mud and seeweed.These isolates were characterized and identified as species of Pseudomonas fluorescens, Serratia marcescens,and Brevibacterium oxydans respectively,based on their physiological and biochemical characteristics and analysis of 16S rDNA sequence.And proteolytic activities of protease from strains 3B,6CW and 15E were researched. |
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| Keywords: | marine bacteria alkaline protease isolation identification |
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