不同大肠杆菌菌株的β－葡萄糖醛酸酶活性及对培植牛黄产量与胆红素含量的影响

将ＥＣＯ１、ＥＣＯ２、ＥＣＯ３和ＥＣＯ－ｍｉｘｔｕｒｅ不同血清型大肠杆菌菌株接种于牛体胆囊培植牛黄．测定接种前后胆汁中β－葡萄糖醛酸酶（β－Ｇ酶）活性及培植１ａ后牛黄产量及胆红素含量．结果表明．ＥＣＯ１、ＥＣＯ２菌株的β－Ｇ酶活性，牛黄胆红素含量比ＥＣＯ３菌株、ＥＣＯ－ｍｉｘｔｕｒｅ菌株明显升高（Ｐ＜０．０１），但牛黄产量明显偏低（Ｐ＜０．０１）；ＥＣＯ－ｍｉｘｔｕｒｅ菌株的β－Ｇ酶活性、牛黄胆红素含量比ＥＣＯ３菌株明显升高（Ｐ＜０．０１），但牛黄产量相近（Ｐ＞０．０５）．综合判断ＥＣＯ－ｍｉｘｔｕｒｅ菌株是培植牛黄的优良菌株．

Effect of β-Glucuronidase Activities Produced by Different Strains of Escherichia coli on the Harvest of Bezoar and Its Bilirubin Content in Bile of Cattle

Four strains of non-pathogenic Escherichia coli,with different serotypes,named as ECO1 ECO2,ECO3 and ECO-mixture,were inoculated into bovine gall bladders to induce bezoars.A few of biles from gall bladders were sampled prior to and post inoculation for the examination of β-glucuronidase activities and the amount of bezoar produced in the gall bldders and its bilirubin contents were examined a year later. The results showed that the levels of β-glucuronidase in the bile and bilirubin in the bezoar inoculated with ECOlor ECO2 were significantly higher than those with ECO3 and ECO-mixture (P<0. 01);Howerer,the amount of bezoar induced by ECO1 and ECO2 were obviously lower than those form ECO3 and ECO-mixture. The β-glucuronidase activities in the bile and bilirubin content in the bezoar inoculated with ECOmixture were significantly higher than those with ECO3 (P<0. 01 ),but the amounts of bezoars induced by these two strains were basically similar(P >0.05).As a conclution,ECO-mixture was considered as the best strain of E.coli for the cultivation of artificial bezoar in this experiment.